Evaluation of fast-track diagnostics and TaqMan array card real-time PCR assays for the detection of respiratory pathogens

Journal of Microbiological Methods
Amanda J DriscollDavid R Murdoch

Abstract

Several commercial assays are now available to detect the nucleic acid of multiple respiratory pathogens from a single specimen. Head-to-head comparisons of such assays using a single set of standard specimens provide additional information about key assay parameters such as sensitivity, specificity and lower limits of detection, and help to inform the decision regarding which method to use. We evaluated two real-time PCR platforms: the Fast-track Diagnostics® (FTD) multiplex respiratory panel and a TaqMan array card (TAC) for simultaneous uniplex detection of multiple respiratory pathogens. Two sets of samples were used to evaluate the assays. One set was created by spiking pooled nasal wash or phosphate buffered saline with specified volumes of known concentrations of virus and/or bacteria. Clinical nasal wash specimens from children with lower respiratory tract illness comprised the other set. Thirteen pathogen targets were compared between the two platforms. Testing with a validation panel of spiked samples revealed a sensitivity of 96.1% and 92.9% for the FTD and TAC assays, respectively. Specificity could not be reliably calculated due to a suspected contamination of the sample substrate. Inter-assay agreement was high (>...Continue Reading

References

Apr 8, 2011·Journal of Clinical Microbiology·Maja KodaniBarry S Fields
Nov 16, 2011·Expert Review of Respiratory Medicine·Richard A Adegbola, Orin S Levine
Apr 17, 2012·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·David R MurdochUNKNOWN PERCH Core Team
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Citations

Mar 5, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·B DavidoA Dinh
Jun 21, 2015·Diagnostic Microbiology and Infectious Disease·Stijn JonckheereHans De Beenhouwer
Apr 14, 2017·Journal of Clinical Microbiology·Clayton O OnyangoBarry Fields
Jun 3, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Amanda J DriscollDavid R Murdoch
Oct 11, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Maureen H DiazJonas M Winchell
Jun 3, 2017·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Daniel R FeikinJ Anthony G Scott
Nov 12, 2020·BMC Infectious Diseases·Keke LiuYongqiang Jiang
Oct 30, 2020·Transplant Infectious Disease : an Official Journal of the Transplantation Society·Alexander ClelandJuraj Petrik
Oct 12, 2016·Microbiology Spectrum·Richard L Hodinka
Sep 2, 2021·The Journal of Infectious Diseases·Mignon du PlessisAnne von Gottberg

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Methods Mentioned

BETA
PCR
nucleic acid extraction
nucleic
Assay

Software Mentioned

Fast
TAC
track

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