PMID: 18193501Jan 15, 2008Paper

Evaluation of filter paper transfer of whole-blood and plasma samples for quantifying HIV RNA in subjects on antiretroviral therapy in Uganda

Journal of Acquired Immune Deficiency Syndromes : JAIDS
Laura WatersMark Nelson

Abstract

Most HIV-infected subjects on antiretroviral therapy (ART) in resource-limited settings do not undergo virologic monitoring. There is an urgent need for cheap, accessible HIV RNA assays for early diagnosis of virologic failure. We investigated filter paper transfer (FPT) of whole blood and plasma as an alternative to standard plasma-based assays for virologic monitoring in Uganda. Whole blood (n = 306) and plasma (n = 218) from 402 subjects established on ART were spotted onto filter paper and transported to Europe for HIV RNA extraction and quantification. These results were compared to a gold standard plasma assay in Kampala. Of 402 ART-treated subjects, 39 (9.7%) had viremia detectable (>500 copies/mL) by local methods. Plasma FPT showed excellent agreement with gold standard, whereas whole blood yielded a large number of false-positive viral loads. This is the first study to investigate the use of FPT in ART-treated subjects and demonstrates that it may provide a practical, reliable method for virologic monitoring in resource-poor settings. Plasma FPT was accurate but requires centrifuge; whole blood produced a high number of false-positive results, but these were low-level. Whole blood may be sufficiently accurate if highe...Continue Reading

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Citations

Sep 25, 2009·The Journal of Antimicrobial Chemotherapy·Asgeir JohannessenAlexandra Calmy
Sep 21, 2011·The Journal of Antimicrobial Chemotherapy·Maria Franca PirilloMarina Giuliano
Jun 4, 2010·Journal of Clinical Microbiology·Katrin SteinmetzerEugen Ermantraut
Feb 20, 2014·The Journal of Infectious Diseases·Maria Raffaella PetraraAnita De Rossi
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May 9, 2012·Enfermedades infecciosas y microbiología clínica·Miguel De Mulder, Africa Holguín
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Dec 8, 2009·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Mauro AndreottiMarina Giuliano
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May 4, 2012·Journal of the International Association of Providers of AIDS Care·Leeann BryantPhilip Keiser
Nov 28, 2014·Journal of Clinical Microbiology·Logan TitchmarshHelen Lee
Jan 21, 2010·AIDS·Asgeir Johannessen
Aug 12, 2010·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Johannes ViljoenUNKNOWN World Health Organization/ANRS 1289 Kesho Bora Study Group
Sep 13, 2008·Expert Review of Molecular Diagnostics·François RouetEric Nerrienet
Aug 14, 2019·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Bernhard KerschbergerEmmanuel Fajardo
Jul 22, 2009·Journal of Acquired Immune Deficiency Syndromes : JAIDS·André Dieudonné MbidaThomas Bourlet
Mar 27, 2020·Frontiers in Microbiology·Edouard TuaillonPhilippe Van de Perre
Sep 26, 2020·Access Microbiology·Abdourahamane YacoubaLassana Sangare

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