Evaluation of five cowside tests for use with milk to detect subclinical ketosis in dairy cows

Journal of Dairy Science
T GeishauserT Duffield

Abstract

The objective of this study was to evaluate the characteristics of five ketone tests for use with milk to detect subclinical ketosis [defined as > 1200 mumol of beta-hydroxybutyrate (BHBA)/L of blood] in dairy cows. The tests studied were the Ketolac BHB strip to detect BHBA (Hoechst, Unterschleissheim, Germany) and four tests based on sodium nitroprusside to detect acetoacetate (Rothera tests) [Ketostix strip (Bayer, Etobicoke, ON, Canada), Bioketone powder (Societé d' Analyses Biopharmaceutiques, Laval, QC, Canada), Ketocheck powder (Great States, St. Joseph, MO), and Utrecht powder (University of Utrecht, Utrecht, The Netherlands)]. Milk samples (n = 529) from 266 cows in 25 Ontario dairy herds were used for this investigation. The Ketolac BHB strip at 50 and 100 mumol of BHBA/L of milk showed sensitivities of 92 and 72%, respectively. This test was more sensitive for subclinical ketosis than were any of the Rothera tests. The sensitivities of the Rothera tests were 43, 33, 28, and 5%, respectively, for Utrecht powder, Bioketone powder, Ketocheck powder, and Ketostix strip. The Rothera tests were highly specific, as was the Ketolac BHB strip at 200, 500, and 1000 mumol of BHBA/L of milk (specificity, > 97%). The prevalence o...Continue Reading

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Citations

May 2, 2009·American Journal of Veterinary Research·Tülay Ileri-BüyükoğluTayfun Güldür
Nov 21, 2017·Journal of Dairy Science·T R OvertonD V Nydam
May 2, 2016·Journal of Dairy Science·E I KaufmanT J DeVries
Jul 20, 2005·Journal of Dairy Science·G UrtonD M Weary
Jun 22, 2015·Journal of Dairy Science·S JorjongV Fievez
May 19, 2009·Journal of Dairy Science·M IwersenW Heuwieser
Mar 21, 2007·Journal of Dairy Science·A P W de RoosG de Jong
Feb 6, 2004·Journal of Dairy Science·C Schlumbohm, J Harmeyer
Jul 4, 2016·Journal of Dairy Science·J E PryceJ B Cole
Feb 9, 2007·Veterinary Research Communications·M SakhaI Taheri
Apr 29, 2015·Journal of Animal Physiology and Animal Nutrition·U NuberR M Bruckmaier
Mar 3, 2020·The Journal of Dairy Research·Maya ZachutLorenzo E Hernández-Castellano
Jan 8, 2013·The Journal of Reproduction and Development·Borjigin SarentonglagaYoshikazu Nagao
Dec 20, 2002·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·A BusatoJ W Blum
Mar 22, 2020·Journal of Dairy Science·Grum GebreyesusGoutam Sahana
Apr 16, 2016·Journal of Animal Physiology and Animal Nutrition·C ViñaF J Diéguez
Aug 13, 2013·Journal of Proteome Research·Matthias S KleinPeter J Oefner
May 18, 2010·Research in Veterinary Science·Huseyin Voyvoda, Hasan Erdogan

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