Evaluation of fluconazole resistance mechanisms in candida albicans clinical isolates from HIV-infected patients in Brazil

Diagnostic Microbiology and Infectious Disease
Gustavo H GoldmanArnaldo L Colombo

Abstract

In this study, we describe resistance mechanisms in fluconazole-resistant isolates of C. albicans isolated from AIDS patients from nine Brazilian hospitals. These mechanisms include the presence of point mutations in the ERG11 gene and overexpression of ERG11, and several genes encoding efflux pumps, as measured by quantitative real-time reverse transcriptase polymerase chain reaction. Several fluconazole-resistant strains had multiple mechanisms of resistance. Four mutations previously described, Y132F, K143R, E266D, and V437I, were identified among the strains, whereas some isolates contained more than one mutation. Fourteen novel mutations were identified. Interestingly, all Brazilian fluconazole-resistant isolates showed homozygosity at mating-type loci (MTL) associated with fluconazole resistance. This is the first comprehensive assessment at molecular level of mechanisms of fluconazole resistance in C. albicans isolates from South America.

References

Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Nov 18, 1998·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·M KolaczkowskiA Goffeau
Feb 23, 1999·Molecular Microbiology·R ManganelliI Smith
Oct 6, 2000·Annual Review of Microbiology·M D De BackerJ Pla
Mar 21, 2002·The Lancet Infectious Diseases·Dominique Sanglard, Frank C Odds
Apr 5, 2002·Microbiology·Tige R RustadTheodore C White
May 23, 2002·Antimicrobial Agents and Chemotherapy·Theodore C WhiteDavid A Stevens
Oct 18, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Sofia Perea, Thomas F Patterson
Jan 8, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Juergen Loeffler, David A Stevens
Mar 26, 2003·Antimicrobial Agents and Chemotherapy·Claude PujolDavid R Soll
Apr 24, 2003·Antimicrobial Agents and Chemotherapy·Adriana M NascimentoDavid S Perlin
Nov 13, 2003·Chembiochem : a European Journal of Chemical Biology·Jochen Wilhelm, Alfred Pingoud

❮ Previous
Next ❯

Citations

Mar 15, 2013·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Ying YingXiaotian Huang
Jan 10, 2012·Photochemistry and Photobiology·Sarit Oriel, Yeshayahu Nitzan
Sep 13, 2006·Antimicrobial Agents and Chemotherapy·Andrew S ChauPaul A Mann
Jan 19, 2006·Clinical Microbiology Reviews·M J EspyT F Smith
Apr 28, 2009·Eukaryotic Cell·Sadri ZnaidiMartine Raymond
Oct 17, 2014·Journal of Chemical Biology·Josie E ParkerSteven L Kelly
Jan 27, 2016·Mycoses·Sarah Santos GonçalvesArnaldo Lopes Colombo
Feb 14, 2013·Revista iberoamericana de micología·Vagner Oliveira CarvalhoGilda M B del Negro
Aug 12, 2009·Fungal Genetics and Biology : FG & B·Joachim Morschhäuser
Sep 24, 2013·Oral Surgery, Oral Medicine, Oral Pathology and Oral Radiology·Paula Volpato SanitáCarlos Eduardo Vergani
Sep 4, 2010·FEMS Immunology and Medical Microbiology·Ezequiel D QuirogaEdgardo N Durantini
Mar 14, 2013·FEMS Yeast Research·Ming-Jie XiangHai-Liang Ge
Apr 30, 2016·Lasers in Medical Science·Cristiane Campos Costa QuishidaAna Cláudia Pavarina
Mar 10, 2015·Research in Microbiology·Jin-Yan LiuMing-Jie Xiang
Apr 12, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Simona Liliana IconaruDaniela Predoi
Feb 9, 2017·Frontiers in Microbiology·Liliana ScorzoniAna M Fusco-Almeida
Aug 12, 2015·Antimicrobial Agents and Chemotherapy·Ana Carolina R SouzaArnaldo L Colombo
May 4, 2005·Yeast
Jul 17, 2008·Mycoses·Paula Volpato SanitáAna Lucia Machado
Mar 7, 2020·Chemical Society Reviews·Kaitlind C HowardSylvie Garneau-Tsodikova

❮ Previous
Next ❯

Related Concepts

Related Feeds

Candidiasis (ASM)

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Candidiasis

Candidiasis is a common fungal infection caused by Candida and it can affect many parts for the body including mucosal membranes as well as the gastrointestinal, urinary, and respiratory tracts. Here is the latest research.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Candida albicans

Candida albicans is an opportunistic, fungal pathogen of humans that frequently causes superficial infections of oral and vaginal mucosal surfaces of debilitated and susceptible individuals. Discover the latest research on Candida albicans here.