PMID: 12751625May 20, 2003Paper

Evaluation of folate conjugate uptake and transport by the choroid plexus of mice

Pharmaceutical Research
Michael D KennedyDor Ben-Amotz

Abstract

Because the choroid plexus (CP) is enriched in cell surface folate receptors, an investigation was initiated to evaluate whether folate receptor-mediated transcytosis might be exploited to deliver folate conjugates into the brain. Balb/c mice were injected with radioactive and fluorescent conjugates of folate to measure and image their uptake by the CP. Retention of a radioactive folate conjugate, folate-diethylenetriaminepentaacetic acid (DTPA)-111In, into the brain of balb/c mice was observed, although repeated injections or prolonged release via an osmotic pump of the compound did not result in increased brain uptake. Uptake of an 125I-labeled anti-folate receptor antibody into the brain was very low, and no competition was observed with unlabeled antibody. Imaging of brain thin-sections and whole brain tissue from a mouse injected with folate-fluorescein revealed strong fluorescence in the CP, but virtually no where else in the brain. Both fluorescence and radioimaging results demonstrate specific uptake of small molecular weight folate conjugates into CP cells of the murine brain, but no significant transport of the molecules across the CSF. Furthermore, no uptake of larger folate-linked proteins by choroid plexus cells is...Continue Reading

Citations

May 17, 2014·Journal of Drug Targeting·Balakumar KrishnamoorthyMarslin Gregory
Aug 28, 2020·Journal of Microencapsulation·Pradeep Kumar BollaJwala Renukuntla
Apr 23, 2004·American Journal of Physiology. Cell Physiology·Ruben M SandovalBruce A Molitoris
Oct 18, 2003·Journal of Biomedical Optics·Michael D KennedyPhilip S Low
Dec 17, 2008·The Journal of Biological Chemistry·Rongbao ZhaoI David Goldman
Dec 19, 2007·Journal of Neurochemistry·Jan B WollackBarton Kamen
Jul 3, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wei HePhilip S Low
Sep 5, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jun YangPhilip S Low
Mar 21, 2017·Chemical Society Reviews·Min GaoLingxin Chen
May 17, 2011·Annual Review of Nutrition·Rongbao ZhaoI David Goldman
Jan 18, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Cristina MüllerMarion de Jong
Sep 2, 2005·Journal of Pharmaceutical Sciences·Andrew R Hilgenbrink, Philip S Low
Jul 22, 2017·Bioconjugate Chemistry·Rachel L MerzelMark M Banaszak Holl

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