PMID: 9431942Feb 12, 1998Paper

Evaluation of four DNA typing techniques in epidemiological investigations of bovine tuberculosis

Journal of Clinical Microbiology
D V CousinsT M Ellis

Abstract

DNA fingerprinting techniques were used to type 273 isolates of Mycobacterium bovis from Australia, Canada, the Republic of Ireland, and Iran. The results of restriction fragment length polymorphism (RFLP) analysis with DNA probes from IS6110, the direct repeat (DR), and the polymorphic GC-rich sequence (PGRS) were compared with those of a new PCR-based method called spacer oligonucleotide typing (spoligotyping) developed for the rapid typing of Mycobacterium tuberculosis (J. Kamerbeek et al., J. Clin. Microbiol. 35:907-914, 1997). Eighty-five percent of the isolates harbored a single copy of IS6110, and 81.5% of these carried IS6110 on the characteristic 1.9-kb restriction fragment. RFLP analysis with IS6110 identified 23 different types, RFLP analysis with the DR probe identified 35 types, RFLP analysis with the PGRS probe identified 77 types, and the spoligotyping method identified 35 types. By combining all results, 99 different strains could be identified. Isolate clusters were frequently associated within herds or were found between herds when epidemiological evidence confirmed animal movements. RFLP analysis with IS6110 was sufficiently sensitive for the typing of isolates with more than three copies of IS6110, but RFLP ...Continue Reading

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Citations

Mar 29, 2000·Journal of Applied Microbiology·E TchernevaL M Herman
Apr 5, 2003·Journal of Applied Microbiology·E Gormley, E Costello
Sep 18, 2003·International Journal of Systematic and Evolutionary Microbiology·Debby V CousinsAmelia Bernardelli
May 7, 2005·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·M S ZaniniM J Zumarraga
Jan 31, 2012·Journal of Molecular Microbiology and Biotechnology·Krishnaveni MohareerSeyed E Hasnain
Feb 9, 2012·Memórias do Instituto Oswaldo Cruz·Patrícia Martins ParreirasPhilip Noel Suffys
Nov 14, 2002·American Journal of Veterinary Research·John B KaneeneKathy Schwartz
Dec 3, 2008·Emerging Infectious Diseases·Keyvan TadayonKen J Forbes
Jan 12, 2000·American Journal of Veterinary Research·F Milian-SuazoM C Torres
Sep 22, 2007·Foodborne Pathogens and Disease·Steven L FoleyRobert D Walker
Oct 12, 1999·Microbiology·M J ZumárragaM I Romano
Dec 8, 2005·Canadian Journal of Microbiology·Laura Cobos-MarínJorge A Gonzalez-y-Merchand
Jul 21, 2005·New Zealand Veterinary Journal·D M Collins
Nov 26, 2010·Journal of Bacteriology·Stefan BergNoel H Smith
Dec 17, 2014·Tuberculosis Research and Treatment·Saman SoleimanpourNader Mosavari
Dec 4, 2012·Vector Borne and Zoonotic Diseases·Vivianne Cambuí Figueiredo RochaJosé Soares Ferreira Neto
Sep 16, 2016·PloS One·Ricardo César Tavares CarvalhoVânia Margaret Flosi Paschoalin
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May 17, 2014·Research in Veterinary Science·E GormleyS Rodriguez-Campos
May 6, 2005·Journal of Clinical Microbiology·G Michael TaylorSimon A Mays
Sep 28, 2001·Journal of Clinical Microbiology·N HaddadB Durand
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Jun 9, 2004·Journal of Clinical Microbiology·Alicia AranazLucas Domínguez
Jan 4, 2001·Journal of Clinical Microbiology·B M Njanpop-LafourcadeN Haddad
May 31, 2002·Journal of Clinical Microbiology·Wolfgang M ProdingerWalter Glawischnig
Jul 18, 2021·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Samuel Kobina Ekuban AcquahDorothy Yeboah-Manu

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