Evaluation of four experimental osteomyelitis infection models by using precolonized implants and bacterial suspensions

Acta Orthopaedica Scandinavica
M MonzónB Amorena

Abstract

Staphylococcus aureus osteomyelitis, a major problem in orthopedic surgery, often involves biofilm bacteria adhering to implants and surrounding bone and tissues. The inadequacy of therapy or immunological surveillance has encouraged studies using animal models which simulate natural osteomyelitic infections, ensure the development of infections and avoid mortality. We evaluated 4 models for infection (8 animals/model) in rats, using stainless-steel implants in tibiae and a very adherent slime-producing bacterial strain. Each animal received: an implant containing a 12 h-biofilm with about 10(6) cfu (Model 1); an implant containing this biofilm and a suspension with about 10(4) cfu (Model 2): a sterile implant and a suspension with about 10(5) cfu (Model 3); or a sterile implant and a suspension with about 10(6) cfu (Model 4). 63 days after surgery we found 100% rat survival, colonization of bone by implant biofilm bacteria in some animals and local, but not systemic infections. Model 1 (but not Models 2-4) reproduced an infection in both, tibiae and implants, most reliably (in 100% of the animals). Model 3 was the least reliable (p < 0.01, 25% infected implants, 12% infected tibiae).

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Citations

Jul 13, 2007·Skeletal Radiology·Cem Nuri AktekinFeza Korkusuz
Jan 6, 2009·Biogerontology·Felícito García-AlvarezRicardo Lozano
Jan 25, 2003·Diagnostic Microbiology and Infectious Disease·Marta MonzónBeatriz Amorena
Mar 25, 2009·Journal of Children's Orthopaedics·Antoine HamelGilles Potel
Sep 8, 2010·Journal of Microbiological Methods·Tom Coenye, Hans J Nelis
Mar 2, 2010·Homo : internationale Zeitschrift für die vergleichende Forschung am Menschen·M DjurićM Roksandic
Jul 6, 2010·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Duane A RobinsonMichael G Conzemius
Feb 16, 2013·Acta Orthopaedica·Niels H SøeHelle Krogh Johansen
Feb 20, 2015·Journal of Materials Science. Materials in Medicine·Ulrika Furustrand TafinMartin Clauss
Jun 6, 2006·Acta Orthopaedica·Thomas KalteisKlaus Lerch
Nov 8, 2017·Journal of Biomaterials Applications·Gracia MendozaManuel Arruebo
Jun 9, 2006·Clinical Orthopaedics and Related Research·Metin Manouchehr EskandariCengiz Yilmaz
Feb 1, 2008·Acta Orthopaedica·Johannes BeckmannKlaus Lerch
May 18, 2006·The International Journal of Artificial Organs·Y H AnC R Arciola
Jan 23, 2020·Journal of Orthopaedic Surgery and Research·Eivind WitsøKåre Bergh
Sep 18, 2017·Biomaterials·Shahed BehzadiMorteza Mahmoudi

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