PMID: 9542931May 23, 1998Paper

Evaluation of four methods for rapid identification of Staphylococcus aureus from blood cultures

Journal of Clinical Microbiology
D J SpeersG L Gilbert

Abstract

The identification of Staphylococcus aureus directly from blood cultures is clinically relevant, but it requires a test that is both rapid and reliable. Previously, biochemical, immunological, tube coagulase, and thermostable-endonuclease methods have shown variable sensitivity and specificity. Testing directly from blood culture broth has not been described for the latex kit Staphaurex Plus (Murex Diagnostics Ltd.), and the modified conventional tests have not been used with the newer, continuously monitored blood culture systems. In addition, the commercial RAPIDEC staph kit (bioMerieux Vitek, Inc.) has been used to detect S. aureus directly from the Vital blood culture system (bioMerieux, Marcy l'Etoile, France), but its performance has not been evaluated with other continuously monitored systems. A total of 201 clinical blood cultures (BACTEC 9240 culture system; Johnston Laboratories, Inc.) in which a Gram stain showed gram-positive cocci resembling staphylococci were evaluated prospectively. The Staphaurex Plus kit, the tube coagulase test, the thermostable-endonuclease test, and the RAPIDEC staph kit were compared. The sensitivities were 23, 92, 85, and 98% and the specificities were 99, 100, 93, and 100%, respectively. ...Continue Reading

References

Sep 1, 1986·Journal of Clinical Microbiology·H Faruki, P Murray
Jun 1, 1985·Journal of Clinical Microbiology·H B Ratner, C W Stratton
Mar 1, 1995·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·O G Brakstad, J A Maeland
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Feb 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·C NeyretJ Etienne

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Citations

Nov 19, 2011·FEMS Microbiology Reviews·Antony CroxattoGilbert Greub
Apr 6, 2011·Expert Review of Molecular Diagnostics·Bernard La Scola
May 25, 2007·Journal of Clinical Microbiology·Qinfang QianJames E Kirby
Dec 8, 2004·Journal of Clinical Microbiology·Laura Lindholm, Hannu Sarkkinen
Feb 8, 2003·Journal of Clinical Microbiology·Kenneth OliveiraHenrik Stender
Sep 6, 2003·Journal of Clinical Microbiology·Kimberle Chapin, Michael Musgnug
Mar 22, 2005·Archives of Medical Research·Roberto Rivera-SánchezMyriam Arriaga-Alba

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