Evaluation of four methods of assigning species and genus to medically important bacteria using 16S rRNA gene sequence analysis

Microbiology and Immunology
Geon ParkDae-Soo Moon

Abstract

The four methods for assigning bacterial species are the Clinical and Laboratory Standards Institute (CLSI), modified CLSI (mCLSI), phylogenetic analysis (PA) and closest match (CM) methods, these are used to identify the genus and species using 16S rRNA gene sequence results. In this study, the results of identification by these four methods of 37 aerobic reference strains, 30 anaerobic reference strains, 15 Acinetobacter reference strains and 167 Acinetobacter clinical strains were compared. The rates of accurate identification to the species level using the CLSI, mCLSI, PA and CM methods were as follows: 24.3, 86.5, 86.5 and 89.2%, respectively, for the 37 aerobic reference strains; 73.3%, 96.7%, 90.0% and 93.3%, respectively, for the 30 anaerobic reference strains; 40.0%, 93.3%, 100% and 93.3%, respectively, for the 15 Acinetobacter reference strains; and 53.9%, 90.4%, 95.8% and 90.4%, respectively, for the 167 Acinetobacter clinical strains. The rates of accurate identification to the genus level using the CLSI, mCLSI, PA, and CM methods were as follows: 91.9%, 91.9%, 94.6% and 91.9%, respectively, for the 37 aerobic reference strains; 100%, 100%, 100% and 100%, respectively, for all of the 30 anaerobic reference strains, ...Continue Reading

References

Jul 25, 2006·Journal of Microbiological Methods·S Mignard, J P Flandrois
Jul 13, 2007·Journal of Clinical Microbiology·J Michael Janda, Sharon L Abbott
Jan 15, 2010·Journal of Clinical Microbiology·Ulrik Stenz JustesenTage Justesen
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Jul 14, 2010·Microbial Ecology·Oksana LukjancenkoDavid W Ussery
Jul 20, 2011·Diagnostic Microbiology and Infectious Disease·Won-Young JinYoung-Jin Park

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Citations

Aug 31, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Mohadeseh Majidzadeh, Mehdi Fatahi-Bafghi

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