Evaluation of four virus recovery methods for detecting noroviruses on fresh lettuce, sliced ham, and frozen raspberries

Journal of Virological Methods
Maija SummaL Maunula

Abstract

Although noroviruses play a significant role in causing foodborne illness in developed countries, no standardised method for detecting noroviruses in foodstuffs is currently available. This study compared four virus recovery methods based on ultrafiltration, immunomagnetic separation, ultracentrifugation and PEG precipitation techniques using identical real-time RT-PCR protocols for detection of RNA in eluates from lettuce, sliced ham and raspberries inoculated artificially with genogroup II norovirus. Noroviruses in all the food source matrices were successfully detected by all four methods. Ultracentrifugation yielded the highest recovery efficiencies in lettuce and ham, whereas PEG precipitation recovered the highest yield of noroviruses from raspberries. The repeatability of the results and the applicability of the methods to all food matrices were best with PEG precipitation, which had average virus recoveries of 19%, 47% and 28% for lettuce, ham and raspberries (viral RNA in dilution 1:10), respectively. In each case, a tenfold dilution of the extracted RNA clearly reduced the level of PCR inhibitors, which were released from raspberries in particular. The results of this study show that the detection of noroviruses in fo...Continue Reading

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Citations

Mar 17, 2015·International Journal of Food Microbiology·Catherine Hennechart-ColletteSylvie Perelle
Feb 21, 2014·International Journal of Food Microbiology·Sandra Martin-LatilSylvie Perelle
May 8, 2013·Food and Environmental Virology·Adriana de Abreu Corrêa, Marize Pereira Miagostovich
Feb 16, 2013·Food and Environmental Virology·Elisabeth MathijsEtienne Thiry
Oct 20, 2015·International Journal of Food Microbiology·Fabiana Gil MelgaçoMarize Pereira Miagostovich
Sep 1, 2015·Journal of Virological Methods·A PerrinC Gantzer
Aug 13, 2015·Food and Environmental Virology·Dina ShahrampourMohebbat Mohebbi
Apr 30, 2016·International Journal of Food Microbiology·Elisabet Marti, Célia Regina Monte Barardi
Mar 1, 2015·International Journal of Food Microbiology·Coralie Coudray-MeunierSylvie Perelle
Oct 5, 2016·Food Microbiology·Catherine Hennechart-ColletteSylvie Perelle
Aug 3, 2013·Journal of Food Protection·Maria RönnqvistLeena Maunula
Mar 31, 2017·Applied Microbiology and Biotechnology·María Dolores Blanco FernándezViviana Andrea Mbayed
Jun 24, 2017·Analytical Chemistry·Nidhi GuptaChris W Diehnelt
Aug 1, 2015·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·L TavoschiD Coulombier
Oct 12, 2017·Food and Environmental Virology·Maija Summa, Leena Maunula
Mar 3, 2021·Food and Environmental Virology·Philippe RaymondLouise Deschênes
Feb 23, 2021·Journal of Food Safety·Alison LacombeVivian C H Wu
Nov 15, 2020·International Journal of Food Microbiology·Catherine Hennechart-ColletteSylvie Perelle
Oct 22, 2019·International Journal of Food Microbiology·Catherine Hennechart-ColletteSylvie Perelle
Jul 8, 2018·Journal of Virological Methods·Isabelle da Silva Luz, Marize Pereira Miagostovich
Jun 17, 2020·Food Microbiology·Catherine Hennechart-ColletteSylvie Perelle
Sep 23, 2021·Applied and Environmental Microbiology·Mathilde Trudel-FerlandJulie Jean

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