PMID: 2108131Apr 1, 1990Paper

Evaluation of freeze-substitution and conventional embedding protocols for routine electron microscopic processing of eubacteria

Journal of Bacteriology
L L Graham, T J Beveridge

Abstract

Freeze-substitution and more conventional embedding protocols were evaluated for their accurate preservation of eubacterial ultrastructure. Radioisotopes were specifically incorporated into the RNA, DNA, peptidoglycan, and lipopolysaccharide of two isogenic derivatives of Escherichia coli K-12 as representative gram-negative eubacteria and into the RNA and peptidoglycan of Bacillus subtilis strains 168 and W23 as representative gram-positive eubacteria. Radiolabeled bacteria were processed for electron microscopy by conventional methods with glutaraldehyde fixation, osmium tetroxide postfixation, dehydration in either a graded acetone or ethanol series, and infiltration in either Spurr or Epon 812 resin. A second set of cells were simultaneously freeze-substituted by plunge-freezing in liquid propane, substituting in anhydrous acetone containing 2% (wt/vol) osmium tetroxide, and 2% (wt/vol) uranyl acetate, and infiltrating in Epon 812. Extraction of radiolabeled cell components was monitored by liquid scintillation counting at all stages of processing to indicate retention of cell labels. Electron microscopy was also used to visually confirm ultrastructural integrity. Radiolabeled nucleic acid and wall components were extracted...Continue Reading

References

Feb 1, 1989·Journal of Electron Microscopy Technique·J Coetzee, C F van der Merwe
Apr 1, 1982·Journal of Bacteriology·H L MobleyS O Langemeier
Nov 1, 1983·Journal of Bacteriology·T J Beveridge, J A Davies
Sep 1, 1981·Archives of Microbiology·H R EbersoldP Lüthy
Aug 1, 1981·Applied and Environmental Microbiology·T J Beveridge, S F Koval
Oct 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·J Spizizen

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Citations

Jan 1, 1992·Microscopy Research and Technique·T S Zorick, C Schooley
Oct 10, 2003·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·K MoriguchiN Ohno
Mar 14, 2019·Canadian Journal of Microbiology·L L Graham, S E Feero
Apr 1, 1996·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·T J Beveridge, J L Kadurugamuwa
Jun 24, 2008·Microscopy Research and Technique·Ghinwa NajaJohn H T Luong
May 1, 1993·World Journal of Microbiology & Biotechnology·L A KirkR I Webb
Nov 20, 2018·Microbes and Environments·Emma J GagenGordon Southam
Jun 24, 2009·Photosynthesis Research·Martin F Hohmann-Marriott, Robert W Roberson
Sep 24, 2004·Astrobiology·Vera S SoinaGalina I El-Registan
Jul 1, 1994·World Journal of Microbiology & Biotechnology·L A KirkH W Doelle
Apr 30, 1998·Critical Reviews in Microbiology·A L Koch
Apr 15, 1993·Microscopy Research and Technique·M T Nicolas, J M Bassot
Dec 16, 2005·Molecular Plant-microbe Interactions : MPMI·Eric KemenRalf T Voegele
Jan 1, 1993·The Journal of Applied Bacteriology·T J Beveridge
Mar 18, 2008·Journal of Bacteriology·Gerhard WannerJörg Overmann
Aug 10, 1999·Journal of Bacteriology·T J Beveridge
May 1, 1995·Journal of Bacteriology·E Hoiczyk, W Baumeister
Dec 1, 1992·Journal of Bacteriology·S Schultze-LamT J Beveridge
Dec 1, 1992·Journal of Bacteriology·A BreitwieserU B Sleytr
Jan 1, 1992·Electron Microscopy Reviews·L L Graham
May 28, 2019·Microbiology Spectrum·Manfred Rohde
Mar 1, 1994·Journal of Bacteriology·L L Graham, T J Beveridge
Dec 1, 1991·Microbiological Reviews·T J Beveridge, L L Graham
Feb 1, 1994·Applied and Environmental Microbiology·K O KonhauserT J Beveridge
Jul 6, 2010·Microbial Pathogenesis·N T Baker, L L Graham

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