Evaluation of Galleria mellonella larvae for measuring the efficacy and pharmacokinetics of antibiotic therapies against Pseudomonas aeruginosa infection

International Journal of Antimicrobial Agents
Lucy HillPeter J Coote

Abstract

The aim of this study was to determine whether Galleria mellonella larvae can be used (i) as an in vivo infection model for Pseudomonas aeruginosa and (ii) for evaluating the pharmacokinetics and efficacy of antipseudomonal antibiotics. Two strains of P. aeruginosa were employed, NCTC 10662 (antibiotic-susceptible) and NCTC 13437 (multidrug-resistant). Larvae were infected with increasing doses of either P. aeruginosa strain to investigate the effect of inoculum size on survival. Subsequently, infected larvae were treated with a range of antibiotics to examine whether these agents were effective against P. aeruginosa infection in vivo and whether the efficacy of these drugs matched the known susceptibilities of each bacterial strain. Larval burden of P. aeruginosa was also determined after infection and treatment with cefotaxime. Pharmacokinetic properties of the antibiotics tested were measured using a well diffusion assay to determine the concentration of antibiotics in larval haemolymph over time. Galleria mellonella larvae were sensitive to P. aeruginosa infection, and increasing inoculum doses of live cells resulted in greater larval mortality. Heat-killed bacteria had no detrimental effect on survival. Antibiotic efficacy...Continue Reading

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Citations

Feb 16, 2015·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Hai-Fei YangJia-Bin Li
Sep 12, 2015·International Journal of Antimicrobial Agents·A ForastieroA Gomez-Lopez
Aug 16, 2016·Journal of Medicinal Chemistry·Bala Kishan GorityalaFrank Schweizer
May 10, 2017·Antimicrobial Agents and Chemotherapy·Zhaojun ZhengEleftherios Mylonakis
Jan 23, 2019·ILAR Journal·Lon V KendallJessica D Ayers
Mar 10, 2019·Molecular & Cellular Proteomics : MCP·Alexander C GrafKatharina Riedel
Mar 4, 2020·Journal of Medical Microbiology·Jennifer Soundy, Darren Day
Jun 22, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Saba ImdadKyeong Kyu Kim
Nov 19, 2019·International Journal of Environmental Health Research·Edit KaszabSándor Szoboszlay
May 30, 2020·International Journal of Molecular Sciences·Alessia BrixAnna Pistocchi
Jun 1, 2019·Molecular & Cellular Proteomics : MCP·Alexander C GrafKatharina Riedel
Apr 2, 2021·Nature Communications·Henrik AlmbladJoe Jonathan Harrison
Jul 14, 2021·Evolution, Medicine, and Public Health·James GurneyMichael E Hochberg

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