Evaluation of geno-toxicity of methyl parathion and chlorpyrifos to human liver carcinoma cell line (HepG2)

Environmental Science and Pollution Research International
Rajashree Patnaik, Rabindra Nath Padhy

Abstract

Insecticides and their residues are known to cause several types of ailments in human body. An attempt had been made to assess digitally the geno-toxicity of methyl parathion (MP) and chlorpyrifos (CP) to in vitro-grown HepG2 cell line, with Hoechst 33342 staining, comet, and micronucleus assays. Additionally, "acridine orange/ethidium bromide" (AO/EB) staining was done for the determination of insecticide-induced cytotoxicity, in corollary. Hoechst 33342 staining of cells revealed a decrease in live cell counts at 8-40 mg/L MP and 15-70 mg/L CP. Moreover, nuclear fragmentations in ranges 8 to 40 mg/L MP and 15 to 70 mg/L CP were recorded dependant on individual doses, increasingly with concomitant increases in comet tail length values. DNA fragmentation index measured in comet assays was 94.3 ± 0.57 at 40 mg/L MP and 93.3 ± 2.08 at 70 mg/L CP. Average micronuclei number was 59.0 ± 2.00 at 40 mg/L MP and 62.6 ± 1.52 at 70 mg/L CP, per 1000 cell nuclei, in micronucleus assay. Minimum inhibitory concentration (MIC) values with AO/EB staining for monitoring cytotoxicity were 4 and 10 mg/L for MP and CP, respectively. Lethal concentration50 (LC50) values were 20.89 mg/L MP and 79.43 mg/L CP in AO/EB staining, for cytotoxicity with ...Continue Reading

References

Apr 1, 1996·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J L MattssonD M Bond
Mar 20, 1997·The Science of the Total Environment·H MallatouT A Albanis
Mar 29, 2000·Environmental and Molecular Mutagenesis·M Kirsch-VoldersA Wakata
Mar 29, 2000·Environmental and Molecular Mutagenesis·R R TiceY F Sasaki
Dec 2, 2000·Nature Neuroscience·R BetarbetJ T Greenamyre
Jan 28, 2004·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Elen S RoslerMelissa K Carpenter
Apr 1, 2004·Drug Discovery Today·Nick Plant
Jul 9, 2004·Environmental Health Perspectives·Robin M WhyattFrederica P Perera
Jun 22, 2006·Toxicology·Ari NakadaiTomoyuki Kawada
Mar 29, 2007·International Journal of Cancer. Journal International Du Cancer·Won Jin LeeMichael C R Alavanja
Jul 3, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Jiguo WuChongyu Lan
Jul 20, 2007·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Walter M A Westerink, Willem G E J Schoonen
Apr 18, 2008·Environmental and Molecular Mutagenesis·Irena HreljacMetka Filipic
Jun 23, 2009·Toxicology·Céline GasnierGilles-Eric Séralini
Oct 24, 2009·Reproductive Toxicology·Amina T FaragAbd El-Khaliek El-Sebae
Aug 31, 2010·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·M J MelgarM A Garcia
May 5, 2011·Interdisciplinary Sciences, Computational Life Sciences·Shakti RathRabindra N Padhy
Jul 26, 2011·Ecotoxicology and Environmental Safety·Meltem Uzunhisarcikli, Yusuf Kalender
Mar 29, 2013·The Journal of Toxicological Sciences·Mansur A SandhuNauman Khalid
Nov 2, 2013·Interdisciplinary Toxicology·Seyed Jalal HosseinimehrMonireh Hedayati
Mar 13, 2014·Archives of Environmental Contamination and Toxicology·Ratana Sapbamrer, Surat Hongsibsong
Apr 2, 2014·BioMed Research International·Noshabah TabassumMuhammad Aqeel Ashraf
Apr 9, 2014·BioMed Research International·Muhammed Alamgir Zaman ChowdhuryAbu Naieum Muhammad Fakhruddin

❮ Previous
Next ❯

Citations

Jul 6, 2016·Archives of Toxicology·M GleiW Schlörmann
Nov 4, 2016·Neurotoxicity Research·Rajashree Patnaik, Rabindra Nath Padhy
Jun 5, 2020·Toxicology and Industrial Health·Anupon TadeeSupatra Porasuphatana
May 13, 2018·Environmental Science and Pollution Research International·Rajashree Patnaik, Rabindra N Padhy

❮ Previous
Next ❯

Related Concepts

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.

© 2022 Meta ULC. All rights reserved