Evaluation of GHb assays in France by national quality control surveys

Diabetes Care
P GilleryA Vassault

Abstract

To evaluate the state of the art concerning GHb assays through analysis of a large-scale quality control survey and to compare the results with those of previous surveys. A lyophilized hemolyzate prepared from human erythrocytes containing a physiological HbA1c level (5.5%) was sent to 3,500 French laboratories in February of 1995 and assayed as a patient's sample under routine conditions. Distribution of values was analyzed from the reported results for each method. The results were compared with the assigned value (acceptable range: +/- 20%) and with the upper value of the reference range currently used. Results were obtained from 2,674 laboratories, among which 39% used cation-exchange chromatography methods, 37.5% affinity chromatography, 16% immunological methods, and 7.5% electrophoresis. The number of laboratories using immunological methods increased from 100 to 400 between 1993 and 1995. The overall interlaboratory coefficient of variation (CV) was 20.2%, with within-method CVs ranging between 3.2 and 29.5%. Method-to-method accuracy varied dramatically, with mean HbA1c values ranging from 4.4 to 8.2%. Results from 75% of the laboratories were comprised in the acceptable range; 88% of them reported a value within the n...Continue Reading

Citations

Oct 9, 2002·Clinica Chimica Acta; International Journal of Clinical Chemistry·Nathalie SchneiderPhilippe Gillery
Apr 15, 2003·Clinica Chimica Acta; International Journal of Clinical Chemistry·Gurdeep S DhattWessel Jenner
Jul 28, 2001·Diabetes Technology & Therapeutics·G S Cembrowski
Aug 6, 2000·Clinical Chemistry and Laboratory Medicine : CCLM·A ChachouP Gillery
Feb 24, 2001·Clinical Chemistry and Laboratory Medicine : CCLM·J TrapéJ M Queraltó
May 19, 2004·Clinical Chemistry and Laboratory Medicine : CCLM·Sandrine Vallée PolneauSimone Bénazeth
Sep 16, 2014·Annales pharmaceutiques françaises·P Gillery
Feb 9, 2006·Scandinavian Journal of Clinical and Laboratory Investigation·W G John
Feb 26, 2010·Diabetes & Metabolism·M MartinP Gillery
Jun 3, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Philippe Gillery
Sep 21, 2012·Clinical Chemistry and Laboratory Medicine : CCLM·Philippe Gillery
Mar 12, 2005·Arquivos brasileiros de endocrinologia e metabologia·Joíza Lins Camargo, Jorge Luiz Gross

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