Evaluation of (GTG)5-PCR for rapid identification of Streptococcus mutans

Antonie van Leeuwenhoek
Pavel SvecIvo Sedlácek

Abstract

Repetitive sequence-based polymerase chain reaction (PCR) fingerprinting using the (GTG)(5) primer was applied for fast screening of bacterial strains isolated from dental plaque of early childhood caries (ECC)-affected children. A group of 29 Gram-positive bacteria was separated into a homogeneous cluster together with Streptococcus mutans reference strains and constituted an aberrant branch after the numerical analysis of (GTG)(5)-PCR fingerprints. Automated ribotyping with EcoRI restriction enzyme (RiboPrinter microbial characterization system) revealed high genetic heterogeneity among the tested group and proved to be a good tool for strain-typing purposes. Further characterization of the studied strains was achieved by extensive phenotyping and whole-cell protein fingerprinting and confirmed all the strains as S. mutans representatives. Obtained results showed rep-PCR fingerprinting with the (GTG)(5) primer to be a fast and reliable method for identification of S. mutans.

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Citations

Jul 3, 2013·Scandinavian Journal of Infectious Diseases·Jiri BenesRoman Pantucek
Nov 26, 2010·Systematic and Applied Microbiology·Pavel SvecDana Nováková
Jul 7, 2010·Journal of Applied Microbiology·T RadimerskyI Literak
Feb 19, 2013·International Journal of Systematic and Evolutionary Microbiology·Petra FrolkováIvan Literák
Aug 23, 2011·International Journal of Systematic and Evolutionary Microbiology·Chun Tao GuGui Cheng Huo
Aug 23, 2011·International Journal of Systematic and Evolutionary Microbiology·Pavel SvecIvo Sedlácek
Aug 6, 2009·International Journal of Systematic and Evolutionary Microbiology·Dana NovákováIvo Sedlácek
Apr 24, 2012·International Journal of Systematic and Evolutionary Microbiology·Ivo SedláčekPavel Švec
Nov 1, 2013·International Journal of Systematic and Evolutionary Microbiology·J KillerO Kofronová
Nov 8, 2011·International Journal of Systematic and Evolutionary Microbiology·R Maarit NiemiKristina Lindström
Jul 4, 2015·Journal of Food Science and Technology·Joana ŠalomskienėJūratė Repečkienė
Aug 11, 2018·International Journal of Systematic and Evolutionary Microbiology·Stanislava KrálováIvo Sedláček
Oct 17, 2019·International Journal of Systematic and Evolutionary Microbiology·Dana NovákováIvo Sedláček
Aug 22, 2020·International Journal of Systematic and Evolutionary Microbiology·Pavel ŠvecIvo Sedláček
Mar 17, 2015·Antonie van Leeuwenhoek·Petra SplichalovaIvan Literak
Dec 19, 2017·The Journal of Veterinary Medical Science·Linda GrillováDavid Šmajs

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