Evaluation of hair roots for detection of methamphetamine and 3,4-methylenedioxymethamphetamine abuse by use of an HPLC-chemiluminescence method

Analytical and Bioanalytical Chemistry
Mitsuhiro WadaKenichiro Nakashima

Abstract

We describe the use of hair roots as a matrix for detection of methamphetamine (MP) and 3,4-methylenedioxymethamphetamine (MDMA) abuse. The concentration of drugs was determined in rat hair roots, hair shafts, and plasma after a single administration of MP or MDMA, by use of an HPLC-peroxyoxalate chemiluminescence (PO-CL) method involving column switching. Plasma and hair roots and shafts were collected from male Wistar rats before and after administration of MP (10 mg kg(-1), i.p.). In addition, the roots and shafts of pigmented and non-pigmented hair of male Lister hooded rats were collected after administration of MDMA (10 mg kg(-1), i.p.). The concentrations of MP and MDMA in plasma and hair were determined by use of the HPLC-PO-CL method, with satisfactory sensitivity and reproducibility. The concentration of MP in hair roots 1-14 days after administration ranged from 0.038 to 0.115 ng mg(-1) (n = 3). By use of the HPLC-PO-CL method, MP could be detected in hair roots for longer (up to 14 days) than it could be detected in conventional biological specimens, for example plasma (~1 day), and MDMA was detected in hair roots from 1 to 10 days after administration. The AUC(1-10) (ng day mg(-1)) for MDMA in roots of non-pigmente...Continue Reading

References

Jun 3, 2004·Forensic Science International : Synergy·Pascal Kintz
Oct 16, 2004·Forensic Science International : Synergy·Amal KaddoumiKenichiro Nakashima
Apr 21, 2006·Clinica Chimica Acta; International Journal of Clinical Chemistry·Fritz Pragst, Marie A Balikova
Jun 17, 2006·Biomedical Chromatography : BMC·Shinichi NakamuraKenichiro Nakashima
Jul 3, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ruri Kikura-HanajiriYukihiro Goda
Sep 12, 2009·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Pinjia MengLiang Zhang
Nov 10, 2009·Forensic Science International : Synergy·Sabina Strano-RossiMaria Jesús Tabernero
Mar 17, 2010·Analytical and Bioanalytical Chemistry·Mitsuhiro WadaKenichiro Nakashima

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Citations

Sep 29, 2019·Chemistry : a European Journal·Laura DelafresnayeChristopher Barner-Kowollik

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