Evaluation of Heat and pH Treatments on Degradation of Ceftiofur in Whole Milk

Frontiers in Veterinary Science
Adriana GarzonRichard Pereira

Abstract

Waste milk feeding practices have been implicated as a potential source for disseminating antimicrobial resistant bacteria among animals and the environment. Two interventions that have shown potential for degrading antimicrobial drugs in milk are heat and pH treatment. The aim of this study was to evaluate the effect of heat and pH treatments on the degradation of ceftiofur and ceftiofur free acid equivalents in milk at concentrations previously found in waste milk on dairy farms by spiking saleable pasteurized whole milk with ceftiofur sodium. Three heat treatments of ceftiofur sodium spiked milk were evaluated for their ability to degrade ceftiofur: 63°C for 30 min (LTLT), 72°C for 15 s (HTST) and 92°C for 20 min (HTLT). Two pH treatments of ceftiofur sodium spiked milk were evaluated: pH 4.0 (LpH) and pH 10 (HpH). Control samples spiked with ceftiofur sodium were kept at room temperature and samples collected at corresponding times for heat and pH treatments. Four treatment replicates were performed for each treatment group. Ceftiofur was quantified in milk samples using liquid chromatography mass spectrometry (LC-MS/MS) and ceftiofur free acid equivalents (CFAE) were measured using high-performance liquid chromatography (H...Continue Reading

References

May 27, 1999·The Journal of Pharmacy and Pharmacology·G SunkaraU B Kompella
Feb 24, 2001·Journal of Dairy Science·J M MusserW A Moats
Oct 26, 2005·Journal of Pharmaceutical and Biomedical Analysis·G A JacobsonC P Cunningham
May 10, 2007·Journal of Agricultural and Food Chemistry·Marta Gratacós-CubarsíMassimo Castellari
Jul 3, 2008·Journal of Food Protection·M A ZorraquinoR Althaus
Oct 23, 2010·Journal of Dairy Science·J A Elizondo-SalazarA J Heinrichs
Feb 23, 2011·Journal of Dairy Science·M RocaM P Molina
Aug 26, 2011·Journal of Agricultural and Food Chemistry·Xiaolin LiMichael Katterhenry
Aug 21, 2013·The Science of the Total Environment·Shannon M MitchellRichard J Watts
Jan 21, 2014·Journal of Dairy Science·R V PereiraL D Warnick
Dec 17, 2014·PloS One·Richard Van Vleck PereiraLorin D Warnick
May 17, 2017·Journal of the American Veterinary Medical Association·Danielle A MzykGeof W Smith
Jan 27, 2017·EFSA Journal·UNKNOWN EFSA Panel on Biological Hazards (BIOHAZ)Lieve Herman

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Citations

Jan 28, 2021·Pathogens·Clair L L FirthAnnemarie Käsbohrer
May 11, 2021·Frontiers in Veterinary Science·Emmanuel OkelloSharif S Aly

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