Abstract
Twenty-three chemicals, differing widely in cytotoxic (hepatotoxic) potency in vivo, were examined to determine their ability to release glutamic-oxaloacetic transaminase (GOT) from hepatocytes isolated by a nonperfusion method from rat liver. The test chemicals were carbon tetrachloride, chloroform, 1,1,2- and 1,1,1-trichloroethane, six bromobenzene analogs, tri-n-butyl tin, chlorpromazine, tetracycline, halothane, phenobarbital, L-ethionine, acetaminophen, thioacetamide, allyl alcohol, ethanol, ascorbic acid, dimethyl sulfoxide, and acetone. In all but two cases--thioacetamide and allyl alcohol--there was a good correspondence between chemicals active in the assay as now performed and those that elevate serum transaminase and cause liver injury on short-term exposure in vivo. These results indicate that with further effort it may be possible to develop an effective, inexpensive, and rapid prescreen to identify drugs and environmental chemicals that are potentially cytotoxic to animals and humans.
References
May 1, 1978·Archives of Biochemistry and Biophysics·H ThorS Orrenius
Apr 1, 1976·Analytical Biochemistry·J R FryJ W Bridges
Jul 1, 1978·Toxicology and Applied Pharmacology·N Stacey, B G Priestly
Jan 1, 1978·Anesthesiology·N H StaceyR C Hall
Feb 1, 1978·Experimental and Molecular Pathology·T D LindstromH Remmer
Jun 1, 1977·Toxicology and Applied Pharmacology·E G ToranzoR A Wiley
Sep 1, 1977·Proceedings of the Society for Experimental Biology and Medicine·C O AbernathyH J Zimmerman
Aug 22, 1977·Biochemical and Biophysical Research Communications·D J Reed, S Orrenius
Aug 1, 1977·Analytical Biochemistry·P BellemannD Mecke
Jun 1, 1976·Environmental Health Perspectives·H J Zimmerman
Jun 1, 1976·Environmental Health Perspectives·J R MitchellJ R Gillette
Aug 1, 1974·Biochemical Pharmacology·H J ZimmermanL Lukacs
Jun 1, 1973·Environmental Health Perspectives·W T Piver
Feb 1, 1974·Toxicology and Applied Pharmacology·H M MalingB B Brodie
Nov 1, 1968·Toxicology and Applied Pharmacology·P J Gehring
Sep 3, 1976·Science·I MuulG Woodard
Citations
Nov 1, 1981·In Vitro·J A Reese, J L Byard
May 15, 1989·Toxicology·I J Boyer
Jan 1, 1989·Toxicology in Vitro : an International Journal Published in Association with BIBRA·N H Stacey
Apr 1, 1983·Journal of Toxicology and Environmental Health·D L StoryD H Gould
May 26, 1998·Environmental Health Perspectives·A Guillouzo
Sep 15, 1983·Toxicology and Applied Pharmacology·C A TysonD H Gould
Jan 1, 1983·Annals of the New York Academy of Sciences·C A McQueen, G M Williams
Jan 1, 1989·Toxicology and Industrial Health·C A Tyson, N H Stacey
Mar 28, 1983·Life Sciences·A P SturdeeD M Crisp
Apr 19, 2019·ACS Medicinal Chemistry Letters·Marina MassaroSerena Riela