PMID: 37196Apr 3, 1979

Evaluation of histochemical observations of activity of acid hydrolases obtained with semipermeable membrane techniques. 3. The substrate specificity of isoenzymes of acid phosphatase in m.gastrocnemius of rabbits

A E MeijerA J Tigges


Three distinct isoenzymes of acid phosphatase have been separated from extracts of m.gastrocnemius of normal and of vitamin E deficient rabbits by gel filtration and polyacrylamide gel electrophoresis. These isoenzymes, termed I, II and III, have molecular weights of: 110,000--130,000, 60,000--78,000 and 12,500--14,500. Isoenzymes I and II split the substrates 4-methylumbelliferyl phosphate and naphthol AS-BI phosphate and the activity is strongly increased in the muscles of vitamin E deficient rabbits. Isoenzyme III splits only 4-methylumbelliferyl phosphate and the activity is not increased in the muscles of vitamin E deficient rabbits. The pH-optimum for isoenzymes I and II is 4.8 and for isoenzyme III 5.5. It has been shown that the histochemical semipermeable membrane technique, using substrate naphthol AS-BI phosphate, is a very reliable technique for demonstrating activity of the isoenzymes I and II in tissue sections. On the other hand, activity of isoenzyme III cannot be demonstrated with this histochemical technique. In pathologically altered muscles, the activity of the isoenzymes I and II is greatly increased whilst the activity of isoenzyme III is not significantly altered.


Mar 1, 1981·The Histochemical Journal·G Huether, V Neuhoff
Sep 1, 1990·Journal of the Neurological Sciences·L Maltz, U Oron
Sep 1, 1979·Histochemistry·M Le HirU Schmidt


Jul 1, 1972·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C W Lin, W H Fishman
Oct 27, 1970·Biochimica Et Biophysica Acta·A KanekoT Onoé
Sep 30, 1969·Biochimica Et Biophysica Acta·D Robinson, P Willcox
Feb 1, 1962·Archives of Biochemistry and Biophysics·A L TAPPELS SHIBKO

Related Concepts

Acid Phosphatase
Vitamin E Assay
Phosphate Measurement
4-methylumbelliferyl phosphate
Limb Structure
Gel Chromatography
Substrate Specificity

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