PMID: 8613673Feb 5, 1996Paper

Evaluation of hollow fiber bioreactors as an alternative to murine ascites production for small scale monoclonal antibody production

Journal of Immunological Methods
L R JacksonN S Lipman

Abstract

The objective of this study was to compare monoclonal antibody production in hollow fiber bioreactor systems and murine ascites to determine the feasibility of the bioreactor system as a potential alternative to the use of mice. Three hybridoma cell lines were grown in each of three different hollow fiber bioreactor systems and in groups of 20 mice. Mice were primed with 0.5 ml pristane intraperitoneally 14 days prior to inoculation of 1X10(6) hybridoma cells. Each mouse was tapped a maximum of three times for collection of ascites. Ascites volumes and daily clinical observations were recorded. Bioreactors were harvested three times weekly for 65 day and were monitored by cell counts, cell viability and media glucose consumption. Time and materials logs were maintained. The total quantity of monoclonal antibody produced in 20 mice versus the mean production for the three different bioreactors in 65 days was as follows: cell line 2B11, 455 mg vs. 168 mg; cell line 3C9, 446 mg vs. 565 mg; and cell line RMK, 997 mg vs. 1023 mg. Mean monoclonal antibody concentration ranged from 4.07 to 8.37 mg/ml in murine ascites, and from 0.71 to 11.10 mg/ml in hollow fiber bioreactor system. Although time and material costs were generally great...Continue Reading

References

Feb 20, 1990·Journal of Immunological Methods·R A KnazekS A Rosenberg
Jul 1, 1988·Laboratory Animals·P WorkmanT C Stephens
Jan 22, 1986·Journal of Immunological Methods·S ReuvenyL Miller
Feb 12, 1986·Journal of Immunological Methods·B R Brodeur, P S Tsang
Mar 15, 1974·Experimental Cell Research·R A KnazekP M Gullino
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J D GroopmanG N Wogan
Jul 6, 1984·Journal of Immunological Methods·B R BrodeurY Larose
Jan 1, 1980·Journal of Immunological Methods·J W Goding
Jan 13, 1995·Journal of Immunological Methods·R JaspertJ L'age-Stehr
May 1, 1986·Biotechnology and Bioengineering·G L AltshulerG Belfort
Nov 1, 1990·Biotechnology and Bioengineering·J M Piret, C L Cooney

❮ Previous
Next ❯

Citations

Nov 13, 2008·Cytotechnology·S M WoodsideJ M Piret
Feb 8, 2003·Hybridoma and Hybridomics·Irena KirmanIlya Trakht
Jun 15, 2005·ILAR Journal·Vincent DewarJean Smal
May 21, 2009·Biotechnology Progress·Christopher J DetzelCornelius F Ivory
Mar 6, 2010·Biotechnology Progress·Christopher J DetzelCornelius F Ivory
Oct 7, 2009·Biotechnology Progress·Christopher J DetzelBernard J van Wie
Jan 1, 2010·Biotechnology Progress·Maria Elisa RodriguesRosário Oliveira
Aug 31, 2000·Annual Review of Biochemistry·D Hilvert
Nov 20, 2012·Toxicon : Official Journal of the International Society on Toxinology·Carlos SevcikHilda Vázquez
Aug 29, 2016·Journal of Immunological Methods·Anna Gall-DebreceniIstvan Kurucz
Apr 1, 1999·Biotechnology and Bioengineering·J E DowdK E Kwok
Jan 30, 2019·NMR in Biomedicine·Marie-France PenetZaver M Bhujwalla
Sep 27, 2005·Alternatives to Laboratory Animals : ATLA·Sandra CoeckeUNKNOWN Second ECVAM Task Force on Good Cell Culture Practice
Jul 10, 2021·The Journal of the Acoustical Society of America·Taigo OyamaKenjiro Takemura
Oct 9, 2007·Biotechnology Advances·Era Jain, Ashok Kumar
Oct 3, 2021·Cold Spring Harbor Protocols·Edward A Greenfield

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.