Evaluation of HPV 16 PCR detection in self- compared with clinician-collected samples in women referred for colposcopy

Gynecologic Oncology
A DaponteI E Messinis

Abstract

A clinical prospective evaluation study was conducted to evaluate PCR detection of high-risk human papillomavirus (HPV) type 16 in self-sampled vaginal compared with clinician-collected cervical specimens. Paired vaginal and cervical specimens were collected from 137 consecutive women referred for colposcopy because of abnormal cervical cytology. In-house and a commercial PCR method for HPV type 16 were used. Self-sampled vaginal HPV 16 detection was compared to histology and physician-collected cervical specimens. Of the 137 patients, 98 had proven abnormal histology and were included in the analysis. Overall, using the cervix HPV detection as reference method, the self-sampled vaginal sample showed sensitivity 91.8%, specificity 96.1% and agreement kappa (kappa) 0.881. Using the histology as reference, all 11 cervical cancer cases were HPV-16-positive in both cervical and vaginal samples, and in 43 high-grade lesions, detection sensitivity in cervix was 72.1% (kappa 0.588) and vagina 67.4% (kappa 0.516). HPV 16 detection did not differ (P=0.27) between clinician-collected cervical and self-sampled vaginal specimens. The self-collected vaginal sample is highly concordant with the physician-collected cervical sample in HPV 16 d...Continue Reading

References

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Citations

Mar 8, 2014·Women's Health·Alexandros DaponteAthanassios Tsakris
Nov 28, 2009·Cancer Causes & Control : CCC·Lindley BarbeeNathalie McKenzie
Nov 1, 2007·Expert Opinion on Medical Diagnostics·Jeong-Hoon Bae, Jong-Sup Park
Aug 22, 2012·International Journal of Cancer. Journal International Du Cancer·Peter J F SnijdersChris J L M Meijer
May 4, 2011·Reviews in Medical Virology·Channa E SchmeinkWillem J G Melchers
Apr 4, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A DaponteI E Messinis
Dec 7, 2018·BMJ : British Medical Journal·Marc ArbynUNKNOWN Collaboration on Self-Sampling and HPV Testing
Sep 12, 2015·Journal of Lower Genital Tract Disease·Eribeth PenarandaNavkiran Shokar

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