Evaluation of HPV Genotyping Assays for Archival Clinical Samples

The Journal of Molecular Diagnostics : JMD
Gabriella Lillsunde LarssonGisela Helenius

Abstract

Human papillomavirus (HPV) testing and genotyping of FFPE tissue samples is important in epidemiological investigations. Here, we compare four different HPV genotyping methods for use in FFPE clinical samples. Comparative testing was performed on 99 samples with a clinical suspicion of HPV. Specimens were analyzed with Anyplex II HPV28 detecting 28 genotypes using real-time PCR and melting curve analysis, CLART HPV2 detecting 35 genotypes using PCR and microarray detection, and MGP5+/6+ consensus primer system together with pyrosequencing. Results were compared to a real-time PCR reference protocol detecting 14 genotypes. In total, 68% of the samples were positive for an HPV genotype using the reference protocol and MGP5+/6+ primer system. Anyplex II HPV28 analysis and CLART HPV2 had 82% and 72% positive samples, respectively. All four methods showed good agreement when comparing the 14 genotypes included in the reference protocol. When evaluating all genotypes, the Anyplex II HPV28 assay and the CLART assay changed the status of the sample (individually or together) from negative with respect to the reference protocol to positive for either a Group 1 (n = 4) or Group 2 (n = 6) genotype. We conclude from this study that for an ...Continue Reading

References

Dec 6, 2002·The American Journal of Pathology·Mythily SrinivasanScott Jewell
Nov 24, 2004·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Thomas McGinnUNKNOWN Evidence-Based Medicine Teaching Tips Working Group
Nov 6, 2007·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Magnus LindhWalter Ryd
Jan 16, 2009·Journal of Clinical Microbiology·Anna Söderlund-StrandJoakim Dillner
Jan 25, 2012·Virchows Archiv : an International Journal of Pathology·Natalie LudygaMichaela Aubele
Aug 15, 2012·International Journal of Radiation Oncology, Biology, Physics·Chun-Chieh WangJi-Hong Hong
Sep 28, 2012·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·Gabriella Lillsunde LarssonMats G Karlsson
Oct 31, 2012·Tropical Medicine & International Health : TM & IH·Cathy NdiayeSilvia de Sanjosé
Dec 5, 2012·Vaccine·Lynette A DennyJoel Palefsky
Feb 14, 2013·Gynecologic Oncology·Gabriella Lillsunde LarssonMats G Karlsson
Aug 10, 2013·International Journal of Cancer. Journal International Du Cancer·Lynette DennyJohannes Schmidt
Aug 13, 2013·European Archives of Oto-rhino-laryngology : Official Journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : Affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·B KoflerR Knecht

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Citations

Dec 2, 2015·Diagnostic Cytopathology·Sule CanberkFernando Schmitt
Oct 31, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Boštjan J KocjanMario Poljak
May 10, 2016·Diagnostic Microbiology and Infectious Disease·Ardashel LatsuzbaiaJoël Mossong
Oct 8, 2016·Acta Obstetricia Et Gynecologica Scandinavica·Sunila ShakyaSolveig Tingulstad
Nov 20, 2016·Current Protocols in Microbiology·Koenraad Van DoorslaerAlison A McBride
Sep 12, 2019·Archives of Pathology & Laboratory Medicine·Francesca RolloMaria Benevolo
Jun 15, 2019·PloS One·Lovisa BergengrenMats G Karlsson
Feb 13, 2016·The International Journal of Biological Markers·Gabriella Lillsunde LarssonGisela Helenius
Apr 19, 2019·BMJ Open Ophthalmology·Ingvild RambergSteffen Heegaard
Aug 21, 2018·Breast Disease·Hadi GhaffariSeyed Hamidreza Monavari
Jul 28, 2017·Cellular Oncology (Dordrecht)·Gabriella Lillsunde Larsson, Gisela Helenius
Mar 31, 2021·Virology Journal·Sara Nicolás-PárragaUNKNOWN RIS HPV TT study groups

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