Evaluation of hypertonic citrate flushing solution for kidney preservation using the isolated perfused rat kidney

Transplantation
M C Bishop, B D Ross

Abstract

The isolated perfused rat kidney has been used to study the effects of cold ischaemia during ice storage. Tissue adenine nucleotide levels in freeze-clamped kidneys were closely correlated with their function after circulation had been reestablished in the perfusion circuit. The ATP content was depleted to a greater extent than total adenine nucleotide after 8 hr of cold ischaemia but both were considerably diminished after 24 hr. The model was also used to compare four solutions which are available clinically for preliminary flushing of organs in the ice storage preservation technique. The results indicated that for periods of 8 and 24 hr of cold ischaemia, renal function and adenine nucleotide content were significantly better maintained with a solution based on hyperosmolar citrate than with Collins, Sacks, or Perfudex solutions. This study confirmed recent observations of the clinical efficacy of citrate solution, while demonstrating how the isolated perfused rat kidney could be used for rapid screening of modifications in preservation techniques.

Citations

Mar 3, 2004·Journal of Pharmacological and Toxicological Methods·Jiping WangSusan Cox
Nov 10, 2000·Toxicon : Official Journal of the International Society on Toxinology·A C NobreH S Monteiro
Aug 8, 2002·Toxicon : Official Journal of the International Society on Toxinology·Alice M C MartinsHelena S A Monteiro
Feb 5, 2003·Toxicon : Official Journal of the International Society on Toxinology·A C L NobreH S A Monteiro
Aug 2, 2003·Toxicon : Official Journal of the International Society on Toxinology·Daniela G de OliveiraHelena S A Monteiro
Oct 8, 2003·Toxicon : Official Journal of the International Society on Toxinology·P E G FacóH S A Monteiro
Sep 2, 1998·Toxicon : Official Journal of the International Society on Toxinology·A M MartinsM C Fonteles
Jan 31, 2007·Journal of Occupational Medicine and Toxicology·Volker UngerDavid A Groneberg
May 23, 2003·Journal of Pharmaceutical Sciences·Christian Grosse-SiestrupDavid A Groneberg
Mar 1, 1983·The Journal of Surgical Research·J Jamart, L Lambotte
Feb 1, 1981·The Journal of Surgical Research·L H Toledo-Pereyra
Dec 1, 1979·The Australian and New Zealand Journal of Surgery·A K HouseA G Sheil
Oct 1, 1979·Research in Experimental Medicine. Zeitschrift Für Die Gesamte Experimentelle Medizin Einschliesslich Experimenteller Chirurgie·R GrundmannH Pichlmaier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Acute viral rhinopharyngitis

Acute viral rhinopharyngitis, also known as "common cold", is an acute, self-limiting viral infection of the upper respiratory tract involving the nose, sinuses, pharynx and larynx. Discover the latest research on acute viral rhinopharyngitis here.

Related Papers

The Journal of Clinical Investigation
Robert W SchrierAmit Mitra
Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association
Michael S Goligorsky
© 2021 Meta ULC. All rights reserved