PMID: 11318408Apr 25, 2001Paper

Evaluation of immobilized metal affinity chromatography for purification of penicillin acylase

Journal of Chromatography. B, Biomedical Sciences and Applications
V Fitton, X Santarelli

Abstract

The aim of this work was to test immobilized metal affinity chromatography (IMAC) for the purification of penicillin acylase. After evaluation of different metals, Cu2+ was selected. Different samples were tested: pure penicillin acylase, industrial clarified feedstock and crude extract. After comparing two eluents, NH4Cl and imidazole, it appeared that although both gave good results for recovery and activity, NH4Cl was a more selective eluent with a higher fold purification than imidazole (4.64 versus 2.04). Moreover, we shown that a multistep gradient of NH4Cl, greatly increased the degree of purification (12.36) compared with the one-step process as control (4.64). In addition, good recovery was obtained (97-100%).

References

Sep 1, 1983·Archives of Biochemistry and Biophysics·J PorathB Granstrand

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Citations

Nov 6, 2001·Journal of Biochemical and Biophysical Methods·V FittonX Santarelli
Jul 31, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yung-Chuan LiuShing-Yi Suen
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Shing-Yi SuenChao-Shuan Chang
Oct 20, 2005·Journal of Bioscience and Bioengineering·Chusnul HidayatToshiomi Yoshida
Apr 17, 2001·Journal of Chromatography. B, Biomedical Sciences and Applications·J SanchezX Santarelli
Jul 9, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·D CoulonX Santarelli
May 23, 2006·International Journal of Biological Macromolecules·Rüstem KeçiliHandan Yavuz

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