Evaluation of immune responses induced by SAG1 and MIC3 vaccine cocktails against Toxoplasma gondii

Veterinary Parasitology
Rui FangJunlong Zhao

Abstract

The aim of this study was to evaluate the immune responses of a SAG1 and MIC3 vaccine cocktail in BALB/c mice. Ninety-six BALB/c mice were randomly divided into eight groups, including three plasmid DNA vaccine groups (pcDNA-MIC3, pcDNA-SAG1, pcDNA-MIC3+pcDNA-SAG1), three recombinant pseudotype baculovirus vaccine groups (BV-G-MIC3, BV-G-SAG1, BV-G-SAG1+BV-G-MIC3) and two control groups (PBS and BV-G-EGFP). All groups were immunized intramuscularly twice at three-week intervals. The production of anti-Toxoplasma gondii lysate antigen (TLA) antibodies, lymphoproliferation, levels of IFN-γ, IL-4 and IL-10 and the survival time were monitored after vaccination. The results showed that immunization of BALB/c mice with MIC3 and SAG1 vaccines stimulated both the cellular and humoral immune responses with the production of anti-T. gondii TLA antibodies. The vaccine cocktails of pcDNA-MIC3+pcDNA-SAG1 or BV-G-SAG1+BV-G-MIC3 induced significantly higher immunogenicity than a single-gene vaccine (P<0.05). Splenocytes from the immunized mice significantly proliferated in response to the TLA and released interferon (IFN)-γ (P<0.05). However, the levels of IL-4 and IL-10 in the sera of the immunized mice were not significantly different from...Continue Reading

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Citations

Jul 22, 2015·Parasites & Vectors·Yanhua Wang, Hong Yin
Oct 8, 2013·Expert Review of Vaccines·Nian-Zhang ZhangXing-Quan Zhu
Aug 27, 2014·Expert Review of Vaccines·Shih-Yeh LinYu-Chen Hu
Aug 17, 2018·Clinical and Experimental Vaccine Research·Masoud ForoutanFatemeh Ghaffarifar
Mar 21, 2017·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Samia E EtewaHowayda S F Moawad
Jan 27, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Samira DodangehFatemeh Rezaei
Oct 11, 2017·Frontiers in Microbiology·Ming PanBang Shen
Jan 21, 2020·Comparative Immunology, Microbiology and Infectious Diseases·Abdol Sattar PaghehAhmad Daryani
Feb 16, 2021·Journal of Proteomics·Rosalba Cruz-MirónRicardo Mondragón-Flores

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