Evaluation of immunization with tachyzoite excreted-secreted proteins in a novel susceptible mouse model (A/Sn) for Toxoplasma gondii

Experimental Parasitology
Thaís A Costa-SilvaVera Lucia Pereira-Chioccola

Abstract

Toxoplasma gondii is an important food-borne parasite transmitted primarily from animals to humans through meat consumption, mainly pork and lamb, as well as through oocysts shed by cats. Infection in humans can cause severe neonatal malformations, ocular complications or encephalitis. Toxoplasmosis infection during pregnancy, especially in sheep, often results in abortion, representing considerable economic loss. The aim of this study was to investigate whether Toxoplasma gondii pooled excreted-secreted antigens (ESA), recovered from infected culture supernatants with tachyzoites used as immunogen, can protect experimental mice against T. gondii infection. For immunization experiments, we evaluated A/Sn inbred mice, a novel susceptible mouse model for T. gondii and a virulent strain (RH) for challenge experiments. The antigen selection was based on those produced by tachyzoites since they are responsible for disseminating the infection as well as stimulating the humoral and cellular immune responses. ESA were recovered from VERO cell-culture supernatants infected with virulent RH strain tachyzoites harvested after 48 h. Groups of 5 female mice were intraperitoneally (i.p.) immunized with 4 doses at 2 week intervals with 20 mic...Continue Reading

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Citations

Aug 21, 2013·Parasitology Research·Yanhua WangMeng Wang
Dec 10, 2009·Future Microbiology·Vera Lucia Pereira-ChioccolaChunlei Su
Jul 8, 2009·Pakistan Journal of Biological Sciences : PJBS·Seyed Ossein AbdollahiGholamhossein Hassanshahi
Dec 4, 2014·Comparative Immunology, Microbiology and Infectious Diseases·Kiumars Norouzpour DeilamiAhad Alizadeh
Mar 1, 2012·Animal Reproduction Science·P I Menzies
Sep 8, 2015·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Hayam Mohamed Ezz EldinLobna Sadek Shash
Feb 7, 2012·Experimental Parasitology·Thaís Alves da Costa-SilvaVera Lucia Pereira-Chioccola
Jun 11, 2015·Jundishapur Journal of Microbiology·Seyed Hossein AbdollahiMohammad Zare-Bidaki
Sep 13, 2011·Diagnostic Microbiology and Infectious Disease·Cristina S MeiraVera L Pereira-Chioccola
May 19, 2012·Experimental Parasitology·Maha M EissaNahed M Baddour
Jun 3, 2009·Expert Review of Vaccines·Józef KurElzbieta Hiszczyńska-Sawicka
Jan 30, 2020·Frontiers in Cellular and Infection Microbiology·Fabiana NakashimaLuiz Carlos de Mattos
Oct 9, 2019·Folia Parasitologica·Junxin XueQuan Wang

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