Evaluation of immunochromatography method for rapid detection of influenza A and B viruses

Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases
Chiharu KawakamiM Imai

Abstract

We have evaluated a new rapid detection kit for influenza A and B viruses, known as the QuickVue Influenza test (Quidel Coporation, USA); which is based on immunochromatography using virus isolates and clinical specimens. Twelve strains of influenza A and B were tested for evaluate the reactivity and detection limits of this test. The QuickVue Influenza test showed a positive result for all twelve strains of influenza virus and a negative result for fourteen different kinds of other respiratory viruses. The detection limits for six strains were 5 to 30 pfu/ml for a cell culture, 1.0 x 10(3) to 6.0 x 10(4) pfu/ml for 1st PCR, 1 to 50 pfu/ml for nested PCR, 3.0 x 10(5) to 6.0 x 10(5) pfu/ml for the QuickVue Influenza test, 1.5 x 10(5) to 1.0 x 10(6) pfu/ml for the Directigen Flu A, and 7.5 x 10(5) to 5.0 x 10(6) pfu/ml for the FLU OIA. Furthermore, the QuickVue Influenza test were clinically evaluated using 92 throat swab specimens collected from patients with influenza-like illnesses. By cell culture, influenza viruses were detected in 49 of the 92 specimens (AH1N1: 20, AH3N2: 7, B: 22); the titers of the influenza viruses were between 2.5 pfu/ml and 7.0 x 10(5) pfu/ml. Compared to cell culture, the QuickVue Influenza test showe...Continue Reading

Citations

Apr 18, 2003·Luminescence : the Journal of Biological and Chemical Luminescence·Komandoor E AchyuthanCraig D Shimasaki
May 2, 2009·Pediatrics International : Official Journal of the Japan Pediatric Society·Masahiro WatanabeToshiaki Ihara
Jun 27, 2006·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·James Mark SimmermanScott F Dowell
May 4, 2004·Journal of Medical Virology·Fabrizio PregliascoUNKNOWN Collaborative Group Influchild
Jul 20, 2004·Journal of Medical Virology·William D RawlinsonIan W Carter
Jun 29, 2002·Journal of Clinical Microbiology·Marilyn S HamiltonKomandoor E Achyuthan

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