Evaluation of immunomodulatory activities of essential oils by high content analysis

Journal of Biotechnology
Marie LangGilles Bedoux

Abstract

Nowadays, research concerning immunomodulatory products are of great interest, particularly in the treatment of inflammatory diseases or the prevention of infectious diseases. These activities are usually evaluated on cell cultures, by tracking different factors requiring dedicated manipulation. Evaluation of the immunomodulatory activities of essential oils and pure compounds using several technics adapted to high content analysis is described in this study. This approach allows a multiparametric evaluation on a single cell batch, in order to obtain an overall response. The developed method is based on the simultaneous evaluation of phagocytosis, production of iNOS and secretion of IL-6, induced by contact of RAW 264.7 cells with LPS. The results highlight the immunomodulatory activities of cinnamon and clove essential oils. They also provide information, particularly concerning the inhibitory activity of mint essential oil, which inhibits the LPS-induced phagocytosis of RAW 264.7 cells by 42%, at 100 μg/ml. This work presents for the first time the adaptation of high content analyses for the monitoring of immunomodulatory activities of essential oils. This protocol could be adjustable to other cell types and supplemented by t...Continue Reading

References

Feb 28, 2002·Annual Review of Immunology·David M Underhill, Adrian Ozinsky
May 20, 2006·The American Journal of Chinese Medicine·Linda S M OoiVincent E C Ooi
Sep 19, 2006·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·E Suzuki, K Umezawa
Sep 18, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Louis Kuoping ChaoShang-Tzen Chang
Nov 13, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·F BakkaliM Idaomar
Apr 22, 2008·BMC Immunology·Annalucia SerafinoPasquale Pierimarchi
Mar 30, 2010·Trends in Biotechnology·Fabian ZanellaWolfgang Link
Dec 17, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria Graça Miguel
Mar 17, 2012·The Journal of Pharmacy and Pharmacology·Tatiana Fernanda BachiegaJosé Maurício Sforcin
Jan 22, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rita de Cássia da Silveira e SáDamião Pergentino de Sousa
Jan 20, 2015·Journal of Agricultural and Food Chemistry·Renato Pérez-RosésSalvador Cañigueral
Mar 12, 2016·Ultrasonics Sonochemistry·Vaiyapuri Subbarayan PeriasamyAli A Alshatwi
Feb 6, 2017·Immunopharmacology and Immunotoxicology·Liza Merly, Sylvia L Smith

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Citations

Nov 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yandong ZhangZhenyu Wang
Dec 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Agnes PeterfalviLaszlo Szereday

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