Evaluation of isolated caprine pancreatic islets cytoarchitecture by laser scanning confocal microscopy and flow cytometry

Xenotransplantation
Homayoun HaniAbas Mazni Othman

Abstract

Pancreatic islets are composed of different hormone-secreting cell types. A finely balanced combination of endocrine cells in the islets regulates intraportal vein secretions and plasma nutrient levels. Every islet cell type is distinguished by its specific secretory granule pattern and hormone content, endocrine and cell signaling mechanisms, and neuronal interactions. The scarcity of pancreatic islet donors for patients with diabetes has caused a considerable interest in the field of islet xenotransplantation. Previous studies have shown that cell arrangement in the pancreatic islets of ruminants differs from that of other mammals; however, caprine islet cytoarchitecture has not yet been comprehensively described. This investigation aimed to characterize caprine islets in regard to better understanding of caprine islet structure and compare with previously reported species, by conducting a detailed analysis of islet architecture and composition using confocal microscopy and immunofluorescence staining for pancreatic islet hormones. After collection and purification of caprine islets with Euro-Ficoll density gradients, islets were considered for viability and functionality procedures with DTZ (dithizone) staining and GSIST (gl...Continue Reading

References

Apr 1, 1990·The Journal of Clinical Investigation·G C Weir, S Bonner-Weir
Nov 1, 1989·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·N J CrowtherI C Green
Feb 1, 1989·Nihon juigaku zasshi. The Japanese journal of veterinary science·H FuruokaC Itakura
Mar 1, 1988·Quarterly Journal of Experimental Physiology : an International Journal of the Physiological Society·S ReddyR B Elliott
Feb 16, 1996·The Journal of Biological Chemistry·B D BennettD W Piston
Mar 1, 1996·Okajimas Folia Anatomica Japonica·T HiratsukaA Kobayashi
Jul 29, 1998·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·G WieczorekE Perentes
Apr 20, 1999·Anatomia, histologia, embryologia·M YukawaS Kitamura
Jun 28, 2003·Diabetes·Troels BockKarsten Buschard
Jun 1, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Marcela BrissovaAlvin C Powers
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Over CabreraAlejandro Caicedo
Feb 27, 2010·Diabetes·Domenico BoscoThierry Berney
Mar 11, 2010·Advances in Experimental Medicine and Biology·Peter In't Veld, Miriam Marichal
Jul 8, 2010·Islets·Abraham KimManami Hara
Feb 15, 2013·Xenotransplantation·Faezeh VakhshitehHomayoun Hani
Mar 22, 2014·Xenotransplantation·Homayoun HaniAbas M Othman
Oct 12, 2014·In Vitro Cellular & Developmental Biology. Animal·Homayoun HaniAbas Mazni Othman

❮ Previous
Next ❯

Citations

May 31, 2016·Xenotransplantation·Christopher Burlak

❮ Previous
Next ❯

Related Concepts

Related Feeds