Evaluation of isolation media for the detection of Bartonella henselae--isolation of Bartonella henselae from domestic cats

Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases
Hidehiro TsuneokaMasato Tsukahara

Abstract

Bartonella henselae is a causative agent of cat scratch disease. We preliminarily tested four media for the bacterial growth, including agar plates with sheep, horse or rabbit blood, and chocolate agar. Of these media, rabbit blood and chocolate agar plate were found to be more excellent for the growth than the medium with sheep or horse blood. Blood samples from 60 domestic cats in Yamaguchi Prefecture were then cultured using 7% rabbit blood agar plates and BACTEC9050 (BD), automated blood culture microbial detection system. B. henselae was isolated from six of the 60 (10%) blood samples. Tiny colonies of B. henselae were visible on the agar medium after one week of culture at 35 degrees C in the 5% CO2 atmosphere. BACTEC 9050 detected B. henselae in one of the 10 blood samples and it took two weeks to detect the bacteria automatically, though gram stain failed to show organisms in the blood culture bottle. In conclusion, rabbit blood or chocolate agar and incubation of agar media more than one week and of BACTEC more than two weeks are recommended for the detection of B. henselae.

Citations

Oct 18, 2016·Internal Medicine·Tsutomu MitoMasatomi Ikusaka
Jun 5, 2014·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Zhanna GeorgievskayaJennifer Picarsic
Jul 7, 2010·FEMS Immunology and Medical Microbiology·Masashi YanagiharaYoshimasa Yamamoto

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cat-scratch disease

Cat-scratch disease, a relatively common infectious disease, is caused by Bartonella henselae and characterized by a fever and regional lymphadenitis. Here is the latest research.

Bartonellosis

Bartonellosis is an infection caused by bacteria in the genus Bartonella and can present as a variety of diseases depending on the organism involved. While asymptomatic infection has been reported, immunocompromised individuals are more susceptible to severe infection. Find the latest research on bartonellosis here.