Evaluation of kahweol and cafestol in coffee tissues and roasted coffee by a new high-performance liquid chromatography methodology

Journal of Agricultural and Food Chemistry
Rafael Carlos Eloy DiasMarta De Toledo Benassi

Abstract

A reverse phase high-performance liquid chromatography (HPLC) method was developed for the simultaneous quantification of kahweol and cafestol in tissues of fresh fruits, leaves, and roasted coffee beans. The best resolution was obtained with isocratic elution of acetonitrile/water (55/45% v/v) and UV detection. A single sample preparation method carried out by direct saponification and extraction with organic solvent was standardized for all matrices. Good recovery (average of 99% for kahweol and 94% for cafestol), repeatability, and linearity were obtained. Detection limits of 2.3 and 3.0 mg/100 g were observed for kahweol and cafestol. The HPLC method was effective in quantifying these diterpenes in the different coffee matrices. The endosperm and perisperm of Coffea arabica cv. IAPAR 59 showed elevated amounts of kahweol as compared to the pericarp and leaves. On the other hand, cafestol was detected in all samples except in leaves from Coffea canephora cv. Apoatā.

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Jul 15, 2011·Omics : a Journal of Integrative Biology·Iris TiskiLuiz Filipe Protasio Pereira
Sep 17, 2013·Molecular Nutrition & Food Research·Franziska FerkSiegfried Knasmüller
Mar 15, 2016·Drug Development and Industrial Pharmacy·Tais A L WagemakerOliveiro Guerreiro Filho
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Oct 15, 2010·Journal of Agricultural and Food Chemistry·Anke KeidelPeter Hildebrandt
Sep 25, 2021·Planta medica·Andrea MontisPierre Van Antwerpen

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