Evaluation of laser ablation microtomy for correlative microscopy of hard tissues

Journal of Microscopy
A Boyde

Abstract

Laser ablation machining or microtomy (LAM) is a relatively new approach to producing slide mounted sections of translucent materials. We evaluated the method with a variety of problems from the bone, joint and dental tissues fields where we require thin undecalcified and undistorted sections for correlative light microscopy (LM) and backscattered electron scanning electron microscopy (BSE SEM). All samples were embedded in poly-methylmethacrlate (PMMA) and flat block surfaces had been previously studied by BSE-SEM and confocal scanning light microscopy (CSLM). Most were also studied by X-yay microtomography (XMT). The block surface is stuck to a glass slide with cyanoacrylate adhesive. Setting the section thickness and levelling uses inbuilt optical coherence tomographic imaging. Tight focusing of near-infrared laser radiation in the sectioning plane gives extreme intensities causing photodisruption of material at the focal point. The laser beam is moved by a fast scanner to write a cutting line, which is simultaneously moved by an XY positioning unit to create a sectioning plane. The block is thereby released from the slide, leaving the section stuck to the slide. Light, wet polishing on the finest grade (4000 grit) silicon c...Continue Reading

References

Jan 1, 1993·Virchows Archiv. A, Pathological Anatomy and Histopathology·J A JayasingheA Boyde
Feb 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R G Erben
Feb 19, 1999·Calcified Tissue International·A BoydeS J Jones
Aug 20, 2003·Journal of Anatomy·Alan Boyde
Apr 27, 2005·European Cells & Materials·Alan BoydeJan Zamecnik
Jul 24, 2010·Neoplasia : an International Journal for Oncology Research·Pierrick G FournierPhilippe Clézardin
Oct 15, 2011·Microscopy Research and Technique·Sim K SinghraoStjohn Crean
May 18, 2012·Endocrinology·J H Duncan BassettGraham R Williams
Jun 8, 2013·Lab on a Chip·Katharina SchimekReyk Horland
Sep 16, 2014·Microscopy Research and Technique·Alan BoydeMichael Doube
Aug 18, 2016·Metallomics : Integrated Biometal Science·Daisy BourassaChristoph J Fahrni
Aug 20, 2016·Journal of Microscopy·B S KowtharapuR F Guthoff
Jul 25, 2017·Journal of Anatomy·Alan BoydeColin Farquharson

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Citations

Jul 4, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Alice GoringClaire E Clarkin
Jun 5, 2021·Calcified Tissue International·Alan Boyde

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