Evaluation of long-term biocompatibility and osteogenic differentiation of graphene nanosheet doped calcium phosphate-chitosan AZ91D composites

Materials Science & Engineering. C, Materials for Biological Applications
Meng ZhaoChangsong Dai

Abstract

To improve the biodegradable performance and osteoinductivity of magnesium (Mg) alloy, a novel graphene nanosheet (GNS) doped micro-arc oxidized-AZ91D (MAO-AZ91D) based calcium phosphate (CaP)-chitosan (CS) (GNS-CaP-CS/AZ91D) composite was fabricated. Their long-term in vitro biocompatibility, osteoinductivity and the related signaling pathway were evaluated. The electrochemical test, Mg concentration and SEM results showed that the corrosion rate of GNS-CaP-CS/AZ91D became much slower, whose corrosion degree with immersion time being 90 d was similar to that of AZ91D being 16 d. Good biocompatibility and non-cytotoxicity were observed during the whole immersion process of GNS-CaP-CS/AZ91D. Extracts from GNS-CaP-CS/AZ91D especially in the early stage (16 days and before) could significantly enhance alkaline phosphatase (ALP) activity, calcium mineral deposition and osteoblast-related genes expression of human bone marrow mesenchymal stem cells (hBMSCs). GNS released from GNS-CaP-CS/AZ91D composites might serve a critical role to induce osteogenic differentiation through ERK/MAPK pathway in the long-term immersion process, while, PI3K/Akt signaling only significantly in the early stage. Moreover, Mg element could promote osteoge...Continue Reading

Citations

Nov 30, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Shaojie DongKaili Lin
Feb 7, 2021·Materials Science & Engineering. C, Materials for Biological Applications·Abudureheman BahatibiekeZixuan Wang
Aug 28, 2021·Journal of Functional Biomaterials·Daniela Medrano-DavidPedronel Araque-Marín
Sep 2, 2021·Journal of Tissue Engineering and Regenerative Medicine·Mengsong WuJun Liu

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