PMID: 8603934Nov 1, 1995Paper

Evaluation of lymphocyte proliferation by immunohistochemistry in the local lymph node assay

Journal of Applied Toxicology : JAT
C Boussiquet-LerouxD Holder

Abstract

A modified version of the local lymph node assay (LLNA) is presented, using bromodeoxyuridine to label proliferating lymphocytes. Cell counting is done on mid-sagittal sections of individual nodes under light microscopy. Two irritants (sodium lauryl sulfate and salicylic acid), four allergens of various sensitizing potential (potassium dichromate, 4-chloroaniline, neomycin sulfate and nickel sulfate) and one chemical of unknown sensitizing potential (ethyl 3-aminobenzoate) were tested either in the short protocol using three daily topical applications and/or in the long protocol with a pre-exposure step under an occluded patch. A weak T cell proliferation was noted with both irritants in the short protocol, but not in the long protocol. Potassium dichromate induced a strong proliferative response in the short protocol. A lesser sensitizing potential was detected for 4-chloroaniline, ethyl 3-aminobenzoate and neomycin sulfate but only in the long protocol. Nickel sulfate was negative in both protocols. The long protocol was the most valuable for weak or moderate sensitizers. Histological examination of nodes ruled out intercurrent processes. The present procedure offers several advantages. The use of a non-isotopic marker enable...Continue Reading

References

Jun 1, 1976·Contact Dermatitis·E Rudzki, A Koslowska
Oct 1, 1992·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·G F GerberickC A Ryan
Feb 1, 1992·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·I Kimber, D A Basketter
Jun 1, 1992·Journal of Applied Toxicology : JAT·E W ScholesS J Waite
Feb 1, 1991·Toxicology Letters·I KimberS J Waite
Apr 1, 1991·Journal of Applied Toxicology : JAT·I Kimber, C Weisenberger
Nov 1, 1990·Contact Dermatitis·I KimberJ Hilton
Feb 1, 1986·Contact Dermatitis·C L Goh, S K Ng
Mar 1, 1969·The Journal of Investigative Dermatology·B Magnusson, A M Kligman
Dec 1, 1983·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·J P GuillotJ M Faccini
Mar 1, 1981·Contact Dermatitis·E Rudzki
Jan 1, 1993·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·D A BasketterP A Botham

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Citations

Jul 19, 2005·Archives of Toxicology·Vohr Hans-Werner, Ahr Hans Jürgen
Jun 1, 2003·Environmental Toxicology and Pharmacology·Jong Kwon LeeHye Young Oh
Oct 12, 2005·Journal of Applied Toxicology : JAT·Masahiro TakeyoshiIan Kimber
Mar 16, 2011·Journal of Applied Toxicology : JAT·Hyun-Ok KuDoug-Young Ryu
May 12, 2004·Regulatory Toxicology and Pharmacology : RTP·K Schneider, Z Akkan
Jun 11, 1999·Journal of Applied Toxicology : JAT·R J DearmanI Kimber
Sep 20, 2002·The Journal of Toxicological Sciences·Akiko SudaKazuichi Nakamura

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