Evaluation of Mass Spectrometry-Based Detection of Panfungal Serum Disaccharide for Diagnosis of Invasive Fungal Infections: Results from a Collaborative Study Involving Six European Clinical Centers

Journal of Clinical Microbiology
Marjorie CornuDaniel Poulain

Abstract

A mass spectrometry (MS) method that detects a serum disaccharide (DS) (MS-DS) was recently described for the diagnosis of invasive fungal infections (IFI). We carried out a European collaborative study to evaluate this assay. Patients with the following IFI were selected according to the availability of sera obtained at about the time that IFI was documented: invasive candidiasis (IC; n = 26 patients), invasive aspergillosis (IA; n = 19), and mucormycosis (MM; n = 23). Control sera originated from 20 neutropenic patients and 20 patients with bacteremia. MS-DS was carried out in blind manner for the diagnosis of IFI. A diagnosis of IC or IA was confirmed by detection of mannan (Man) or galactomannan (GM), respectively, associated with detection of (1,3)-β-d-glucan (BDG) in both infections. MM was detected by quantitative real-time PCR (qPCR). All tests discriminated sera from patients with IC from sera from control subjects with bacteremia (P ≤ 0.0009). For IC, the MS-DS sensitivity and specificity were 51% and 87%, respectively. MS-DS complemented the high specificity of Man monitoring. All tests discriminated sera from IA patients from sera from neutropenic controls (P ≤ 0.0009). For IA, MS-DS sensitivity and specificity were...Continue Reading

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Citations

Feb 9, 2020·Intensive Care Medicine·Elie AzoulayUNKNOWN Nine-i Investigators
Mar 7, 2020·Journal of Clinical Microbiology·David Terrero-Salcedo, Margaret V Powers-Fletcher
Oct 2, 2019·Journal of Fungi·Amir ArastehfarTeun Boekhout
Nov 10, 2020·Translational Research : the Journal of Laboratory and Clinical Medicine·Liesbeth VanherpUwe Himmelreich

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Methods Mentioned

BETA
PCR
Biopsy
bronchoalveolar
lavage

Software Mentioned

GraphPad Prism

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