Evaluation of meiotic spindles in thawed oocytes after vitrification using polarized light microscopy

Fertility and Sterility
Chun-Kai ChenYung-Kuei Soong

Abstract

To investigate the efficacy of the PolScope on imaging the spindle morphology in oocytes at the metaphase II stage before vitrification and after thawing. In vitro study. University infertility clinic and academic research laboratory. Oocytes at the metaphase II stage that were obtained from superovulating mice were vitrified and then thawed. Morphological features of the spindle in oocytes were evaluated by both the PolScope and immunofluorescent staining. Using the PolScope, the morphological features of the spindle of intact thawed oocytes were undetected by 3 hours of thawing in only 25% of cases. Most of the spindle images were recognized during the first hour of observation. Additionally, the statistical analysis of agreement of spindle morphology by both the PolScope and fluorescent staining showed a weighted Kappa value of 0.70, indicating good agreement. Oocytes with good spindle morphology verified by the PolScope before vitrification had a higher survival rate of intact oocytes after thawing compared with those with poor or undetected spindle images. The morphological features of the spindle in oocytes evaluated by the PolScope before freezing and after thawing are significantly correlated with those assessed by immu...Continue Reading

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Citations

Apr 10, 2009·Zygote : the Biology of Gametes and Early Embryos·Zengyan WangFangfang He
Jan 21, 2009·Reproduction, Fertility, and Development·Ching-Chien ChangZsolt Peter Nagy
May 26, 2009·Human Reproduction·J J BromfieldD F Albertini
Feb 10, 2011·Human Reproduction·Javier I GarcíaLuis Noriega-Hoces
May 3, 2011·Human Reproduction Update·Markus MontagHans van der Ven
May 10, 2011·Reproductive Biomedicine Online·Ching-Chien ChangZsolt Peter Nagy
Jan 5, 2011·Reproductive Biomedicine Online·Ching-Chien ChangZsolt Peter Nagy
Dec 8, 2007·Reproductive Biomedicine Online·M G LarmanD K Gardner
Mar 25, 2008·Fertility and Sterility·Claudia Messias GomesGary D Smith
Feb 6, 2007·Fertility and Sterility·James W AkinJeffrey Boldt
Oct 31, 2012·Reproduction in Domestic Animals = Zuchthygiene·J N CaamañoE Gómez
Jun 15, 2012·Reproduction in Domestic Animals = Zuchthygiene·C DíezE Gómez
Jul 16, 2010·Reproduction in Domestic Animals = Zuchthygiene·J N CaamañoE Gómez
Nov 17, 2015·Iranian Red Crescent Medical Journal·Neda Abedpour, Farzad Rajaei
May 10, 2015·Journal of Assisted Reproduction and Genetics·Gao ShanshanChen Zi-Jiang
Jan 22, 2014·Reproductive Biomedicine Online·Luna MaoFan Jin
Mar 25, 2014·Reproductive Biomedicine Online·Jason E Swain
Aug 29, 2009·Reproductive Biomedicine Online·E SereniA Borini

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