Evaluation of metanephric maturation in a human fetal kidney explant model

In Vitro Cellular & Developmental Biology. Animal
D G Matsell, T Bennett

Abstract

We have developed a unique human fetal kidney explant model to study the role of the insulin-like growth factor (IGF) system in metanephric development. Kidneys from 10-18 wk gestation human abortuses were maintained in serum-free conditions and defined medium, which was shown to support the induction and differentiation of the viable metanephric blastema. Histologically the tissue remained viable to 192 h of serum-free culture, while metanephric differentiation, reflected by a shrinking nephrogenic zone and the formation of maturing S-shape and glomerular forms, was accelerated and occurred between 48 and 96 h. In the nephrogenic zone, a significant decrease in IGF-II gene expression occurred, which reflected the differentiation of the metanephric blastema cell mass, IGF-II expression persisted, however, in the expanded interstitial mesenchyme. With differentiation over 48 h an increase in IGFBP-2 and WT1 gene expression by Northern blot analysis occurred, and was localized by in situ hybridization to the differentiating glomerular epithelial cell mass. Analysis of the explant-conditioned media by Western ligand blot demonstrated an increase in the rate of IGF binding protein (IGFBP)-2 peptide production by the differentiating...Continue Reading

References

Feb 1, 1992·The American Journal of Physiology·B A DavisW F Boron
Jul 1, 1987·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·L Saxén, H Sariola
Jan 1, 1987·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·L LaitinenL Saxén
Jun 24, 1994·Science·J J LetterioA B Roberts
Aug 27, 1993·Cell·J A KreidbergR Jaenisch
Jan 1, 1997·Journal of the American Society of Nephrology : JASN·D G MatsellV K Han

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