Evaluation of metastatic niches in distant organs after surgical removal of tumor-bearing lymph nodes

BMC Cancer
Jinhua ZhengTetsuya Kodama

Abstract

Surgical removal of primary tumors can promote the incidence of tumor metastasis. However, molecular mechanisms underlying this process remain unclear. We inoculated tumor cells expressing luciferase gene  into subiliac lymph node (SiLN) of the MXH10/Mo-lpr/lpr mice. The tumor-bearing SiLNs were surgically removed at a certain period of time after inoculation. In vivo bioluminescence imaging system and histological staining revealed metastasis in lung, proper axillary lymph node (PALN) and liver. The lung metastasis rate in SiLN removal groups was significantly higher than in the control group using Fisher exact test. Mann-Whitney U-test indicated that the luciferase-positive tumor cells in the lung and liver were significantly higher than in the control groups. The lung samples in SiLN removal groups had strong expression of lysine oxidase (LOX). Moreover, the number of CD11b+ cells in the lung and liver in the SiLN removal groups was significantly increased, which was positively correlated with LOX expression level. In addition, the condition of LOX and CD11b in liver was similar to lung. In the SiLN surgical removal groups, the matrix metalloproteinase (MMP)-2 and VEGFA expression in the lung tissues was significantly higher...Continue Reading

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Citations

Dec 1, 2018·Cancer Science·Ariunbuyan SukhbaatarTetsuya Kodama
Dec 7, 2019·International Journal of Cancer. Journal International Du Cancer·Hans RaskovIsmail Gögenur
Nov 7, 2019·Scientific Reports·Ariunbuyan SukhbaatarTetsuya Kodama
Sep 5, 2021·Trends in Cell Biology·Sarah K Deasy, Neta Erez

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Methods Mentioned

BETA
dissection
surgical resection
surgical stress

Software Mentioned

SPSS
Image Pro Plus

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