Evaluation of method for secondary DNA typing of Mycobacterium tuberculosis with pTBN12 in epidemiologic study of tuberculosis.

Journal of Clinical Microbiology
Z YangM D Cave

Abstract

Secondary fingerprinting of Mycobacterium tuberculosis DNA with a probe containing the polymorphic GC-rich repetitive sequence present in pTBN12 has been found to have greater discriminating power than does fingerprinting with the insertion sequence IS6110 for strains carrying few copies of IS6110. To validate the use of pTBN12 fingerprinting in the molecular epidemiology of tuberculosis, M. tuberculosis isolates from 67 patients in five states in the United States and in Spain were fingerprinted with both IS6110 and pTBN12. Epidemiologic links among the 67 patients were evaluated by patient interview and/or review of medical records. The 67 isolates had 5 IS6110 fingerprint patterns with two to five copies of IS6110 and 18 pTBN12 patterns, of which 10 were shared by more than 1 isolate. Epidemiologic links are consistently found among patients whose isolates had identical pTBN12 patterns, whereas no links were found among patients whose isolates had unique pTBN12 patterns. This suggests that pTBN12 fingerprinting is a useful tool to identify epidemiologically linked tuberculosis patients whose isolates have identical IS6110 fingerprints containing fewer than six fragments.

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Citations

Jul 2, 2002·American Journal of Respiratory and Critical Care Medicine·Stephen E WeisPeter F Barnes
Feb 15, 2014·BioMed Research International·Tomasz JagielskiJacek Bielecki
Feb 27, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Richard E NettlesTimothy R Sterling
Apr 16, 2003·Journal of Applied Microbiology·E KandumaS H Gillespie
Apr 13, 2005·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Marcos BurgosCharles L Daley
Oct 17, 2006·Clinical Microbiology Reviews·Barun MathemaBarry N Kreiswirth
Nov 28, 2002·Emerging Infectious Diseases·Kashef IjazM Donald Cave
Nov 14, 2002·American Journal of Veterinary Research·John B KaneeneKathy Schwartz
Jan 25, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Samuel J McConkeyThomas C Bailey
Sep 17, 1999·Journal of Clinical Microbiology·R W WilsonP F Barnes
Mar 9, 2004·Journal of Clinical Microbiology·Madalene RichardsonRobin M Warren
May 23, 1998·Journal of Clinical Microbiology·Z YangM D Cave
Nov 18, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·R DurmazM D Cave

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