Evaluation of MetriGenix custom 4D arrays applied for detection of breast cancer subtypes

BMC Cancer
Aslaug Aamodt MuggerudAnne-Lise Børresen-Dale

Abstract

Previously, a total of five breast cancer subtypes have been identified based on variation in gene expression patterns. These expression profiles were also shown to be associated with different prognostic value. In this study tumour samples from 27 breast cancer patients, previously subtyped by expression analysis using DNA microarrays, and four controls from normal breast tissue were included. A new MetriGenix 4D array proposed for diagnostic use was evaluated. We applied MetriGenix custom 4D arrays for the detection of previously defined molecular subtypes of breast cancer. MetriGenix 4D arrays have special features including probe immobilization in microchannels with chemiluminescence detection that enable shorter hybridization time. The MetriGenix 4D array platform was evaluated with respect to both the accuracy in classifying the samples as well as the performance of the system itself. In a cross validation analysis using "Nearest Shrunken Centroid classifier" and the PAM software, 77% of the samples were classified correctly according to earlier classification results. The system shows potential for fast screening; however, improvements are needed.

References

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Citations

Dec 21, 2007·Molecular Biotechnology·Gabriel Betanzos-CabreraKenneth L Beattie
Nov 15, 2007·Journal of the National Cancer Institute·Lara LusaMarco A Pierotti
Oct 14, 2009·Seminars in Thoracic and Cardiovascular Surgery·Raphael Bueno

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Methods Mentioned

BETA
20
biopsy
electrophoresis
chip
chips

Software Mentioned

Metrisoft
PAM
MetriGenix
Avadis Prophetic

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