PMID: 15334839Sep 1, 2004Paper

Evaluation of microbial culture of pooled fecal samples for detection of Mycobacterium avium subsp paratuberculosis in large dairy herds

American Journal of Veterinary Research
Saraya TavornpanichSharon K Hietala

Abstract

To evaluate sensitivity of microbial culture of pooled fecal samples for detection of Mycobacterium avium subsp paratuberculosis (MAP) in large dairy herds and assess the use of the method for estimation of MAP prevalence. 1,740 lactating cows from 29 dairy herds in California. Serum from each cow was tested by use of a commercial ELISA kit. Individual fecal samples were cultured and used to create pooled fecal samples (10 randomly selected fecal samples/pool; 6 pooled samples/herd). Sensitivity of MAP detection was compared between Herrold's egg yolk (HEY) agar and a new liquid culture method. Bayesian methods were used to estimate true prevalence of MAP-infected cows and herd sensitivity. Estimated sensitivity for pooled fecal samples among all herds was 0.69 (25 culture-positive pools/36 pools that were MAP positive). Sensitivity increased as the number of culture-positive samples in a pool increased. The HEY agar method detected more infected cows than the liquid culture method but had lower sensitivity for pooled fecal samples. Prevalence of MAP-infected cows was estimated to be 4% (95% probability interval, 2% to 6%) on the basis of culture of pooled fecal samples. Herd-level sensitivity estimate ranged from 90% to 100% a...Continue Reading

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Citations

May 30, 2009·Preventive Veterinary Medicine·Catherine A AlinoviChing Ching Wu
Aug 28, 2007·Preventive Veterinary Medicine·Saraya TavornpanichIan A Gardner
Sep 26, 2006·Veterinary Microbiology·Graeme J EamensRichard J Whittington
Jan 24, 2006·Preventive Veterinary Medicine·Claudia Muñoz-ZanziWesley Johnson
Jul 26, 2011·Veterinary Medicine International·J A Fernández-SilvaM Bülte
Jun 23, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Roy D BerghausIan A Gardner
Sep 3, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Sharif S AlyIan A Gardner
Mar 14, 2014·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ken Steen PedersenOystein Angen
Feb 16, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·N Beth HarrisJanet B Payeur
Jan 10, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mark E ArnoldGeoff C Pritchard
Jun 24, 2017·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Henry S LaneWendy L McDonald
Nov 4, 2005·Journal of the American Veterinary Medical Association·Steven H HendrickTodd F Duffield
Mar 19, 2008·Animal Health Research Reviews·Locksley L McV MessamIan A Gardner

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