Evaluation of microdialysis sampling of aqueous humor for in vivo models of ocular absorption and disposition

Journal of Pharmaceutical and Biomedical Analysis
K D RittenhouseG M Pollack

Abstract

The dynamics of beta-adrenergic-associated reductions in aqueous humor production for treatment of elevated intraocular pressure are not well understood. In particular, the relationship between ocular pharmacokinetics and pharmacodynamics has yet to be established. This study was undertaken to develop a procedure for examining the ocular absorption and disposition of topically administered ophthalmic beta-adrenergic antagonists in individual animals. Dogs were anesthetized with isoflurane and a microdialysis probe was implanted in the anterior chamber of one eye and perfused with 0.9% saline at a rate of 2 microliters min-1. 3H-propranolol was administered by intracameral injection or topically. Each dog received intracameral and topical propranolol, in alternate eyes on separate days, in a randomized cross-over fashion. Microdialysis probe effluent was collected every 5 min for > or = 2.5 h; concentrations of propranolol were determined by liquid scintillation spectroscopy and were corrected for probe recovery of the substrate as determined by in vivo retrodialysis (approximately 46%) to estimate aqueous humor concentrations. In separate experiments in rabbits, microdialysis probes were implanted in each eye. 3H-propranolol wa...Continue Reading

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Citations

Jul 29, 1999·Biomedical Chromatography : BMC·C S Chaurasia
May 4, 2010·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Indu Pal KaurShilpa Kakkar
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Dec 8, 2000·Advanced Drug Delivery Reviews·K D Rittenhouse, G M Pollack
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Jun 23, 2010·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Kumar G JanoriaAshim K Mitra
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Jan 5, 2013·Current Opinion in Ophthalmology·Daniel W Pita-Thomas, Jeffrey L Goldberg
Feb 5, 2013·International Journal of Pharmaceutics·Jike SongDamei Liu
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Apr 4, 2014·Biological & Pharmaceutical Bulletin·Irhan Ibrahim Abu HashimHidetoshi Arima
Dec 23, 2009·Pharmaceutical Development and Technology·Ghada Abdelbary

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