Evaluation of microparticle chemically bonded reversed-phase packings in the high-pressure liquid chromatographic analysis of nucleosides and their bases

Journal of Chromatography
R A Hartwick, P R Brown

Abstract

The reversed-phase partition mode of high-pressure liquid chromatography was used for the analysis of seven of the naturally occurring nucleosides and their bases. With microparticle chemically bonded packings, nucleosides and their bases can be quantitatively determined in the presence of nucleotides in 30 min with high sensitivity, accuracy, and reproducibility. Peaks in chromatograms of cell extracts were identified by absorbance ratios and enzymatic peak shift methods. Applications of this technique to biochemical studies are reported.

References

Aug 29, 1973·Journal of Chromatography·K W StahlD Bojanowski
Apr 1, 1974·European Journal of Biochemistry·R P Singhal
Jan 29, 1968·Biochimica Et Biophysica Acta·M Carrara, G Bernardi
Apr 12, 1972·Biochimica Et Biophysica Acta·R P Singhal, W E Cohn
Aug 26, 1971·Biochimica Et Biophysica Acta·S D EhrlichG Bernardi
Oct 15, 1969·Analytical Biochemistry·F J Kull, M Soodak
Feb 1, 1970·Analytical Biochemistry·J L Hoffman
Dec 23, 1971·Journal of Chromatography·F MurakamiH Hatano
May 1, 1967·Analytica Chimica Acta·J F Huber, J A Hulsman

Citations

Nov 15, 1980·Analytical Biochemistry·C D Lothrop, M Uziel
Nov 24, 1982·Clinica Chimica Acta; International Journal of Clinical Chemistry·M ZakariaB E Barker
Jan 1, 1989·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·D VannoniR Marcolongo
Dec 21, 1984·Journal of Chromatography·S Hjertén, D Yang
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·R C WillisJ E Seegmiller
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T S Chan
Sep 1, 1982·The Proceedings of the Nutrition Society·D Giesecke, W Tiemeyer
Feb 24, 1984·Nucleic Acids Research·C DesgrangesE De Clercq
Jul 10, 1981·Nucleic Acids Research·J M BogdenE Bresnick
May 1, 1983·The Journal of Clinical Investigation·W R OsborneD C Dale
Sep 1, 1984·The Journal of Clinical Investigation·J G Puig, I H Fox
Mar 1, 1987·The Journal of Clinical Investigation·F A MateosI H Fox
Jan 1, 1986·Free Radical Research Communications·W SiemsG Gerber
May 1, 1991·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·N LaudignonJ V Aranda
May 1, 1990·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·N LaudignonJ V Aranda
Dec 28, 2018·Nucleosides, Nucleotides & Nucleic Acids·David Perrett
Jan 1, 1982·Cancer Chemotherapy and Pharmacology·J F Kuttesch, J A Nelson
Jun 2, 1989·Journal of Chromatography·C W Gehrke, K C Kuo

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