Evaluation of microplate Alamar blue assay for drug susceptibility testing of Mycobacterium avium complex isolates

Diagnostic Microbiology and Infectious Disease
J Daisy Vanitha, C N Paramasivan

Abstract

Fifty-one clinical isolates and 5 clarithromycin-resistant mutants of Mycobacterium avium complex (MAC) were tested for their susceptibility to clarithromycin by microplate Alamar blue assay (MABA). The susceptibility results were compared with the results obtained by the BACTEC 460 method. All clinical isolates were susceptible, while all mutants were resistant to clarithromycin by BACTEC. Eighty-six percent of the clinical isolates were susceptible by MABA, and one of the resistant mutants was misclassified as susceptible by this method. The overall agreement between MABA and BACTEC was 86%, indicating the usefulness of MABA in drug susceptibility testing of MAC.

References

Mar 1, 1995·Antimicrobial Agents and Chemotherapy·R C CookseyJ T Crawford
Nov 1, 1993·Antimicrobial Agents and Chemotherapy·L HeifetsJ Vanderkolk
May 1, 1995·Clinics in Dermatology·F Portaels
Jul 18, 2000·Tubercle and Lung Disease : the Official Journal of the International Union Against Tuberculosis and Lung Disease·M A JamalY Kashiwabara
Oct 10, 2002·Clinics in Chest Medicine·Theodore K Marras, Charles L Daley
Oct 10, 2002·Clinics in Chest Medicine·Michael D Iseman

❮ Previous
Next ❯

Citations

Oct 12, 2010·Organic & Biomolecular Chemistry·Ram Shankar UpadhayayaJyoti Chattopadhyaya
Jul 31, 2012·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·João V de AssisSergio A Fernandes
Mar 19, 2011·Chemical Biology & Drug Design·Silvia H CardosoMarcus V N de Souza
Nov 15, 2011·Chemical Biology & Drug Design·Cristiane F Da CostaMarcus V N De Souza
Aug 26, 2011·Analytical Biochemistry·Rabeay Y A Hassan, Ursula Bilitewski
Jun 21, 2015·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Joana Darc Souza ChavesMauro Vieira de Almeida
Feb 8, 2011·European Journal of Medicinal Chemistry·Wilson CunicoMaria C S Lourenço
Mar 31, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·I B RamisA Martin
Mar 17, 2012·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Melby Mendoza-AguilarOscar Rojas-Espinosa
Jan 29, 2013·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Lulu LianKanglin Wan
Aug 25, 2009·Tuberculosis·Kathryn E A LougheedRoger S Buxton
Oct 7, 2014·Chemical Research in Toxicology·Eva CagideLuis M Botana
Nov 30, 2005·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ricardo F RosenbuschLorraine J Hoffman
Oct 18, 2015·Scientia pharmaceutica·Emerson T da SilvaSolange M S V Wardell
Mar 13, 2014·Archiv der Pharmazie·Laura N F CardosoMarcus V N de Souza
Jun 25, 2008·Archiv der Pharmazie·Amit TrivediViresh Shah
Apr 20, 2010·Organic & Biomolecular Chemistry·Ram Shankar UpadhayayaJyoti Chattopadhyaya
Jul 23, 2019·Anti-infective Agents·L SivasankerreddyR Nageswarao
Jun 14, 2020·Molecular Diversity·Hymavathi VeeravarapuMurali Krishna Kumar Muthyala
Jul 23, 2011·Journal of Medicinal Chemistry·Nubia BoechatCarolina C P Costa
Feb 20, 2010·Carbohydrate Research·Maurício F SaraivaMauro V de Almeida
Oct 12, 2010·European Journal of Medicinal Chemistry·Raoni S B GonçalvesAdilson D da Silva

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.