Evaluation of MicroRNA Delivery In Vivo

Methods in Molecular Biology
Rikki A M BrownPeter J Leedman

Abstract

MicroRNAs (miRNAs) are a family of short noncoding RNA molecules that fine-tune expression of mRNAs. Often their altered expression is associated with a number of diseases, including cancer. Given that miRNAs target multiple genes and "difficult to drug" oncogenes, they present attractive candidates to manipulate as an anti-cancer strategy. MicroRNA-7 (miR-7) is a tumor suppressor miRNA that has been shown to target oncogenes overexpressed in cancers, such as the epidermal growth factor receptor (EGFR) and the nuclear factor-κ B subunit, RelA. Here, we describe methods for evaluating systemic delivery of miR-7 using a lipid nanoparticle formulation in an animal model. The microRNA is delivered three times, over 1 week and tissues collected 24 h after the last injection. RNA and protein are extracted from snap frozen tissues and processed to detect miRNA distribution and subsequent assessment of downstream targets and signaling mediators, respectively. Importantly, variability in efficiency of miRNA delivery will be observed between organs of the same animal and also between animals. Additionally, delivering the microRNA to organs other than the liver, particularly the brain, remains challenging. Furthermore, large variation in ...Continue Reading

Citations

Oct 23, 2018·The FEBS Journal·Hanane Hadj-Moussa, Kenneth B Storey
Nov 7, 2019·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Qinghai YouWenmei Li
Jan 4, 2020·Journal of Biomedical Science·Rui Rui WongChit Laa Poh
Feb 6, 2020·Frontiers in Pharmacology·Dong HanJianfei Nao
Jan 27, 2021·Biochimica Et Biophysica Acta. Molecular Basis of Disease·Jianguo WuLi Wang

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