PMID: 11909839Mar 23, 2002Paper

Evaluation of MIDITECH automated colorimetric MIC reading for antimicrobial susceptibility testing

The Journal of Antimicrobial Chemotherapy
Rainer GattringerApostolos Georgopoulos

Abstract

The MIDITECH colorimetric susceptibility test with automated reading is a modification of the standard broth microdilution method that uses a 3-(4,5-dimetylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) dye for detecting viable bacteria. The method can be applied to non-fastidious aerobic Gram-negative bacteria, staphylococci and Enterococcus faecalis. To assess the reliability of this method, we compared susceptibility data obtained by this test with standard NCCLS microdilution assay results. For this purpose, 15 antibiotics and a well characterized set of 527 Gram-negative and Gram-positive bacterial isolates collected and stored at the Division of Infectious Diseases and Chemotherapy (Vienna General Hospital, Austria), yielding 5751 organism-antibiotic combinations, were analysed in duplicate. The overall essential agreement (+/-1 log(2) dilution) between the MIDITECH and NCCLS methods was 96.18 +/- 0.67%. The colorimetric assay compared with the reference method produced MICs < or = 2 log(2) dilutions and > or = 2 log(2) dilutions in 2.34% and 1.48% comparisons, respectively. For 326 Gram-negative bacteria, the absolute interpretative agreement of both methods ranged from 87.12% for ampicillin-sulbactam to 99.85% for...Continue Reading

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Citations

Dec 31, 2002·Folia Microbiologica·A Hostacká, L Klokocníková
Sep 30, 2006·Folia Microbiologica·K HorosováV Kmet
Aug 4, 2010·Folia Microbiologica·V Kmet, E Piatnicová
Aug 22, 2007·International Journal of Antimicrobial Agents·Daniela OhlasovaMilan Niks
Sep 15, 2004·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Jaroslav Timko
Dec 23, 2017·Antonie van Leeuwenhoek·Veronika KadlicekovaHana Drahovska
May 3, 2019·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Livia Handrova, Vladimir Kmet
Aug 9, 2006·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Peter K Linden, David L Paterson
Sep 21, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xu ChenJie Liu
Jul 3, 2021·Microorganisms·Lívia KarahutováDobroslava Bujňáková
Jul 22, 2020·Journal of Infection and Public Health·Katarína ČurováLeonard Siegfried

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