Evaluation of molecule-microbe interactions with capillary electrophoresis: procedures, utility and restrictions

Electrophoresis
Alain BerthodD W Armstrong

Abstract

Understanding the interactions between molecules and living organisms is of paramount importance for the evaluation of pharmaceutical activity, chemical toxicity and all manner of microbiological studies. The capability of capillary electrophoresis (CE) in the evaluation of molecule-microbe interactions is examined in the present paper. The fundamental chemical concept of the binding or association constant for molecular systems measured in free solution is discussed for biological systems where microorganisms uptake or associate with molecules from their environment. The heterogeneity of the living organisms must be understood and accounted for including differences related to semantics such as concentration units and the nature of the associations between two entities and large differences in the size and number of microorganisms as compared to molecules. Finally, the added complexity and even inhomogeneity of a cell compared to most molecular systems must be considered and possibly controlled. The binding of specific molecules to viruses is discussed. CE can be utilized to quickly determine if a molecule binds very strongly or not at all to a cell (i.e., a binary yes/no answer). This could be useful for initial high-throughp...Continue Reading

References

Sep 11, 1992·Journal of Chromatography·J C KraakH Poppe
Jan 1, 1980·Annals of the New York Academy of Sciences·W K Joklik
Jan 1, 1980·Annals of the New York Academy of Sciences·W K Joklik
Jan 1, 1995·Methods in Enzymology·H LefflerC Svanborg
Dec 10, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·D S Hage, S A Tweed
Feb 28, 1998·The Journal of Biological Chemistry·Y Chien, A L Cheung
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·N H HeegaardN A Guzman
Jan 7, 2000·Analytical Chemistry·D W ArmstrongD J Westenberg
Jul 6, 2000·Analytical Chemistry·S N Krylov, N J Dovichi
Sep 7, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·N S TanJ L Ding
Sep 7, 2000·The Journal of Biological Chemistry·P J SimpsonM P Williamson
Nov 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·M N LiangG M Whitesides
May 31, 2001·Electrophoresis·K L Rundlett, D W Armstrong
Nov 1, 1946·Journal of the American Chemical Society·G SCATCHARD

❮ Previous
Next ❯

Citations

Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Meera J Desai, Daniel W Armstrong
Jun 23, 2010·Colloids and Surfaces. B, Biointerfaces·V SborosV Koutsos
Dec 7, 2007·Electrophoresis·Carl I D Newman, Greg E Collins
Sep 22, 2012·Electrophoresis·Ewa KłodzińskaBogusław Buszewski
Oct 1, 2010·Electrophoresis·Hadewych HalewyckBart Rombaut
Jul 23, 2003·Analytical Biochemistry·Pertti J Viskari, Christa L Colyer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.