Evaluation of mRNA markers for estimating blood deposition time: Towards alibi testing from human forensic stains with rhythmic biomarkers

Forensic Science International. Genetics
Karolina LechManfred Kayser

Abstract

Determining the time a biological trace was left at a scene of crime reflects a crucial aspect of forensic investigations as - if possible - it would permit testing the sample donor's alibi directly from the trace evidence, helping to link (or not) the DNA-identified sample donor with the crime event. However, reliable and robust methodology is lacking thus far. In this study, we assessed the suitability of mRNA for the purpose of estimating blood deposition time, and its added value relative to melatonin and cortisol, two circadian hormones we previously introduced for this purpose. By analysing 21 candidate mRNA markers in blood samples from 12 individuals collected around the clock at 2h intervals for 36h under real-life, controlled conditions, we identified 11 mRNAs with statistically significant expression rhythms. We then used these 11 significantly rhythmic mRNA markers, with and without melatonin and cortisol also analysed in these samples, to establish statistical models for predicting day/night time categories. We found that although in general mRNA-based estimation of time categories was less accurate than hormone-based estimation, the use of three mRNA markers HSPA1B, MKNK2 and PER3 together with melatonin and corti...Continue Reading

References

Jul 1, 1971·The Journal of Clinical Endocrinology and Metabolism·E D WeitzmanL Hellman
Dec 12, 1980·Science·A J LewyS P Markey
Jan 1, 1994·International Journal of Legal Medicine·H MikamiK Haga
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Apr 23, 2002·Nature·Kai-Florian StorchCharles J Weitz
May 23, 2002·Cell·Satchidananda PandaJohn B Hogenesch
Aug 21, 2003·Forensic Science International : Synergy·Jane Juusola, Jack Ballantyne
Nov 10, 2004·Journal of Biological Rhythms·Hitoshi Okamura
Jan 26, 2006·International Journal of Psychophysiology : Official Journal of the International Organization of Psychophysiology·William R Lovallo
May 12, 2006·Chronobiology International·Josephine Arendt
Feb 6, 2007·Biochemical and Biophysical Research Communications·Hiroyuki FukuyaMitsuhiro Yokoyama
Sep 18, 2007·Journal of Forensic Sciences·Jane Juusola, Jack Ballantyne
Mar 14, 2008·Neuroscience Research·Hiroaki KusanagiKazuo Mishima
Jul 3, 2008·International Journal of Legal Medicine·Dmitry ZubakovManfred Kayser
Mar 13, 2009·Current Biology : CB·Fan LiuManfred Kayser
Mar 17, 2009·PloS One·Katarzyna BozekHanspeter Herzel
Apr 4, 2009·PLoS Genetics·Michael E HughesJohn B Hogenesch
Jun 3, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yoichi MinamiHiroki R Ueda
Jun 3, 2009·Nature Reviews. Endocrinology·George P Chrousos
Sep 24, 2009·Bioinformatics·Darya ChudovaPadhraic Smyth
Oct 29, 2009·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·N Cermakian, D B Boivin
Dec 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maren KellerBert Maier
Apr 27, 2010·International Journal of Legal Medicine·Katrin AckermannManfred Kayser
Aug 24, 2010·Forensic Science International : Synergy·Rolf H BremmerMaurice C G Aalders
Nov 12, 2010·International Journal of Legal Medicine·Akihiko KimuraToshikazu Kondo
Feb 2, 2011·Journal of Forensic Sciences·Clint HampsonSuzzanne McColl
Feb 19, 2011·Nature Reviews. Genetics·Manfred Kayser, Peter de Knijff
Jun 11, 2011·Forensic Science International : Synergy·Szilvia Arany, Susumu Ohtani
Aug 27, 2011·Forensic Science International : Synergy·Rolf H BremmerMaurice C G Aalders
Mar 1, 2012·Forensic Science International. Genetics·Alexander LindenberghTitia Sijen
Apr 10, 2012·Annual Review of Neuroscience·Jennifer A MohawkJoseph S Takahashi
Aug 25, 2012·Forensic Science International. Genetics·Susan WalshManfred Kayser
Aug 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Takeya KasukawaHiroki R Ueda
Dec 21, 2012·International Journal of Legal Medicine·Adrián OdriozolaMaría T Zarrabeitia
Jan 11, 2013·Physiological Reviews·Kristin Eckel-Mahan, Paolo Sassone-Corsi
Feb 26, 2013·PloS One·Fernanda Sampaio-SilvaRicardo Silvestre
Feb 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Carla S Möller-LevetDerk-Jan Dijk

❮ Previous
Next ❯

Citations

Jan 27, 2018·BMC Genomics·Soheil YousefiPeter A C 't Hoen
Aug 7, 2017·International Journal of Legal Medicine·Karolina LechManfred Kayser
Feb 22, 2017·ELife·Emma E LaingDerk-Jan Dijk
May 11, 2017·Proceedings of the National Academy of Sciences of the United States of America·Rikuhiro G Yamada, Hiroki R Ueda
Jul 5, 2019·Forensic Science International. Genetics·Cornelius CourtsWalther Parson
Feb 18, 2021·International Journal of Legal Medicine·Jan Euteneuer, Cornelius Courts
Apr 28, 2021·Journal of Proteome Research·Donatella AielloAnna Napoli
Jul 7, 2021·Journal of Photochemistry and Photobiology. B, Biology·Alexis WeberIgor K Lednev

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.