Evaluation of multivalent Leptospira fluorescent antibody conjugates for general diagnostic use

Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
D A MillerC A Kirkbride

Abstract

Four lots of conjugate were evaluated for optimal dilution and degree of fluorescence produced with reference cultures and bovine and porcine leptospira isolates. One lot that uniformly produced better fluorescence was evaluated for sensitivity and specificity with reference cultures, isolates, culture-positive tissues, and 13 other bacterial species. Further evaluation of the conjugates was done with bovine, porcine, and ovine specimens submitted to a diagnostic laboratory. Leptospires were detected with the fluorescent antibody test (FAT) in 9 of 21 culture-positive bovine kidneys and were detected in diluted cultures when present at concentrations of 10(2)-10(3) organisms/ml. With the exception of Treponema hyodysenteriae, FAT's of other bacterial cultures produced minimal fluorescence or were negative. Positives were characterized by moderate to brilliant fluorescence of typical cell forms, and most nonspecific fluorescence was eliminated with a flazo-orange counterstain. The results indicated that the FAT utilizing multivalent conjugates could be used successfully as an additional method for diagnosis of leptospira infections.

Citations

Mar 17, 2004·Journal of Insect Physiology·Bruce A WebbKaren J McDowell
Feb 19, 2011·Avian Pathology : Journal of the W.V.P.A·Róbert GlávitsAdám Dán
Jul 1, 1996·Equine Veterinary Journal·H KindeJ T Dowe
Jul 1, 1996·Equine Veterinary Journal·P M HoganM R Fitzgerald
Aug 13, 2014·Diseases of Aquatic Organisms·Qingzhong WuLori Schwacke
Feb 2, 2006·Avian Pathology : Journal of the W.V.P.A·C S NemesL Dencso
Jul 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J M DonahueM A Wilson
Jan 1, 1995·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J M DonahueC L Rigsby
Oct 25, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Nadia A B AntoniassiDavid Driemeier
Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·C A Kirkbride
Apr 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J C NietfeldR D Neiger
Oct 1, 1989·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·E C HodginF Lozano
Jan 23, 2010·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Sreekumari RajeevCharles A Baldwin
Mar 1, 2013·Expert Review of Clinical Immunology·Stefan SchreierWannapong Triampo
Apr 1, 1991·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·J M DonahueJ K Donahue

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