Evaluation of murine thymocyte stimulation using a defined culture medium

Immunobiology
F KristensenA L de Weck

Abstract

The ability of RPMI 1640 enriched with FCS or AATSZ (L-alanine, BSA, human transferrin, zinc chloride, and sodium selenite) to support mitogen-induced activation (G0-G1 shift) and proliferation (G1-S shift) of thymocytes has been investigated. The two culture media were found to be equally supportive. In terms of viability, differences were detected in the number of recovered viable cells, but this could be related to alterations in adherent properties, rather than viability of the cells. For the examination of a PHA-induced proliferation, IL-1-containing suppernatants, deriving from normal or induced peritoneal macrophages, were prepared. The supportive capacity of these preparations showed no significant difference between AATSZ and FCS. Despite the excellent supportive capacity for the mitogen-stimulated thymocyte cultures, the AATSZ medium was not able to support all established cell lines tested. A T cell (MOLT 4F) and a macrophage cell line (SK 1) grew equally well in AATSZ- and FCS-enriched medium, but a B cell (U 266) and a null-cell line (Reh) did not proliferate at all. When cells from the latter two lines were cultured in AATSZ medium, they did not complete the RNA-synthesis required for DNA-synthesis, as judged by c...Continue Reading

References

Dec 1, 1977·European Journal of Immunology·M Burger
Jul 1, 1972·The Journal of Experimental Medicine·I GeryB H Waksman
Jun 1, 1980·The Journal of Experimental Medicine·K A SmithM F Favata
Feb 1, 1980·European Journal of Immunology·E L Larsson, A Coutinho

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Citations

Mar 1, 1983·Veterinary Immunology and Immunopathology·O Barta
Apr 1, 1984·Cellular Immunology·D O SlausonA L de Weck

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